Tested Applications
| Positive WB detected in | HeLa cells |
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24797-1-AP targets C19orf61 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14965 Product name: Recombinant human C19orf61 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 145-520 aa of BC008869 Sequence: PVYQIQNRGMGTAAPAAMDPVVGQAKLLPPERMKHSIKLVDDQMNWCDSAIEYLLDQTDVLVVGVLGLQGTGKSMVMSLLSANTPEEDQRTYVFRAQSAEMKERGGNQTSGIDFFITQERIVFLDTQPILSPSILDHLINNDRKLPPEYNLPHTYVEMQSLQIAAFLFTVCHVVIVVQDWFTDLSLYRFLQTAEMVKPSTPSPSHESSSSSGSDEGTEYYPHLVFLQNKARREDFCPRKLRQMHLMIDQLMAHSHLRYKGTLSMLQCNVFPGLPPDFLDSEVNLFLVPFMDSEAESENPPRAGPGSSPLFSLLPGYRGHPSFQSLVSKLRSQVMSMARPQLSHTILTEKNWFHYAARIWDGVRKSSALAEYSRLLA Predict reactive species |
| Full Name | chromosome 19 open reading frame 61 |
| Calculated Molecular Weight | 520 aa, 58 kDa |
| Observed Molecular Weight | 57-65 kDa |
| GenBank Accession Number | BC008869 |
| Gene Symbol | C19orf61 |
| Gene ID (NCBI) | 56006 |
| RRID | AB_2879731 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H0W8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for C19orf61 antibody 24797-1-AP | Download protocol |
| IP protocol for C19orf61 antibody 24797-1-AP | Download protocol |
| WB protocol for C19orf61 antibody 24797-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C19orf61 (SMG9) Polyclonal antibody, catalog number 24797-1-AP, has 1 total citation. The citation appears in the journal Heliyon (2024), where the antibody was used for Western blot in human samples. The associated research field is glioma biology, specifically investigating SMG9 as a prognostic biomarker correlated with ferroptosis and immune infiltrates in glioma patients.
| Species | Application | Title |
|---|---|---|
Heliyon SMG9 is a novel prognostic-related biomarker in glioma correlating with ferroptosis and immune infiltrates
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