Tested Applications
| Positive WB detected in | mouse kidney tissue, HeLa cells, PC-3 cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
24930-1-AP targets C2orf47 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21518 Product name: Recombinant human C2orf47 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 1-291 aa of BC017959 Sequence: MALAARLLPQFLHSRSLPCGAVRLRTPAVAEVRLPSATLCYFCRCRLGLGAALFPRSARALAASALPAQGSRWPVLSSPGLPAAFASFPACPQRSYSTEEKPQQHQKTKMIVLGFSNPINWVRTRIKAFLIWAYFDKEFSITEFSEGAKQAFAHVSKLLSQCKFDLLEELVAKEVLHALKEKVTSLPDNHKNALAANIDEIVFTSTGDISIYYDEKGRKFVNILMCFWYLTSANIPSETLRGASVFQVKLGNQNVETKQLLSASYEFQREFTQGVKPDWTIARIEHSKLLE Predict reactive species |
| Full Name | chromosome 2 open reading frame 47 |
| Calculated Molecular Weight | 291 aa, 33 kDa |
| Observed Molecular Weight | 38 kDa |
| GenBank Accession Number | BC017959 |
| Gene Symbol | C2orf47 |
| Gene ID (NCBI) | 79568 |
| RRID | AB_2879804 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWC4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C2orf47, also named matrix AAA peptidase interacting protein 1 (MAIP1), is a 291 amino-acid protein that localizes in the Mitochondrion. C2orf47 is involved in protecting EMRE, a component of the single calcium complex (MCU), from proteolysis by assisting membrane insertion.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for C2orf47 antibody 24930-1-AP | Download protocol |
| WB protocol for C2orf47 antibody 24930-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C2orf47 Polyclonal antibody (Cat# 24930-1-AP) has 1 total citation. It was cited in Nature Cell Biology in a study investigating small heat shock proteins as molecular chaperones in the mitochondrial intermembrane space. The research field encompasses cell biology, protein chaperone function, and mitochondrial biology. The antibody was applied in Western blotting for both human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Small heat shock proteins operate as molecular chaperones in the mitochondrial intermembrane space |
