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What's the best dilution for CDC14B in ?
Tested Applications
| Positive WB detected in | SMMC-7721 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25187-1-AP targets CDC14B in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18227 Product name: Recombinant human CDC14B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 349-498 aa of BC156666 Sequence: PGSVIGPQQQFLVMKQTNLWLEGDYFRQKLKGQENGQHRAAFSKLLSGVDDISINGVENQDQQEPEPYSDDDEINGVTQGDRLRALKSRRQSKTNAIPLTVILQSSVQSCKTSEPNISGSAGITKRTTRSASRKSSVKSLSISRTKTVLR Predict reactive species |
| Full Name | CDC14 cell division cycle 14 homolog B (S. cerevisiae) |
| Calculated Molecular Weight | 498 aa, 57 kDa |
| Observed Molecular Weight | 56 kDa |
| GenBank Accession Number | BC156666 |
| Gene Symbol | CDC14B |
| Gene ID (NCBI) | 8555 |
| RRID | AB_2879949 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O60729 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for CDC14B antibody 25187-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CDC14B Polyclonal antibody (Cat# 25187-1-AP) has 1 total citation. The cited publication appeared in Scientific Reports (2016), investigating the roles of APC/C(CDC20) and APC/C in embryonic development in Artemia sinica. The research field is cell cycle regulation and embryonic development.
| Species | Application | Title |
|---|---|---|
Sci Rep APC/C(CDC20) and APC/C play pivotal roles in the process of embryonic development in Artemia sinica. |
