Tested Applications
| Positive WB detected in | MCF-7 cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25269-1-AP targets WRN in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | Werner syndrome, RecQ helicase-like |
| Calculated Molecular Weight | 162 kDa |
| Observed Molecular Weight | 162 kDa |
| GenBank Accession Number | NM_000553 |
| Gene Symbol | WRN |
| Gene ID (NCBI) | 7486 |
| RRID | AB_2879997 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14191 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Werner syndrome ATP dependent helicase (WRN) ,also named as RECQ3 or RecQ protein like 2, is a 1432 amino acid protein, which contains one HRDC domain, one helicase C-terminal domain, one helicase ATP-binding domain and 3'-5' exonuclease domain. WRN localizes in the nucleoplasm and belongs to the helicase family. WRN plays a central role in the repair of methylation-induced DNA damage and has an helicase activity necessary to prevent dramatic telomere loss during DNA replication. WRN gene mutation has an association with Werner syndrome and colorectal cancer.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for WRN antibody 25269-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The WRN Polyclonal antibody (Cat# 25269-1-AP) has 1 citation to date. It was cited in Molecular Carcinogenesis (2025), in a study investigating how LINC02562 promotes lung cancer progression by regulating NTHL1-dependent DNA damage repair mechanisms. The antibody was used for Western blot in human and mouse samples. The associated research field is cancer biology, specifically lung cancer and DNA damage repair pathways.
| Species | Application | Title |
|---|---|---|
Mol Carcinog LINC02562 Promotes Progression of Lung Cancer by Regulating NTHL1 Dependent DNA Damage Repair Mechanisms |
