Tested Applications
| Positive IHC detected in | human thymus tissue, human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25285-1-AP targets CCL25/TECK in IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17994 Product name: Recombinant human CCL25 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 24-150 aa of BC130561 Sequence: QGVFEDCCLAYHYPIGWAVLRRAWTYRIQEVSGSCNLPAAIFYLPKRHRKVCGNPKSREVQRAMKLLDARNKVFAKLHHNTQTFQAGPHAVKKLSSGNSKLSSSKFSNPISSSKRNVSLLISANSGL Predict reactive species |
| Full Name | chemokine (C-C motif) ligand 25 |
| Calculated Molecular Weight | 150 aa, 17 kDa |
| GenBank Accession Number | BC130561 |
| Gene Symbol | CCL25/TECK |
| Gene ID (NCBI) | 6370 |
| RRID | AB_2880008 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15444 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCL25, also known as Thymus-Expressed Chemokine (TECK), is a small chemokine belonging to the CC subfamily, first isolated from mouse thymus in 1997. Its official full name is "C-C motif chemokine ligand 25". CCL25 is mainly expressed in the thymus and small-intestinal epithelium, and to a lesser extent by vascular endothelial cells and other parenchymal tissues. It selectively attracts dendritic cells, thymocytes, and activated macrophages via binding to its high-affinity G-protein-coupled receptor CCR9, while showing no chemotactic activity on peripheral blood lymphocytes or neutrophils. Through this CCR9-CCL25 axis it orchestrates T-cell development in the thymus and guides CCR9⁺ lymphocytes-especially IgA-producing plasma cells and intra-epithelial lymphocytes-to the small intestine, thus participating in mucosal immunity and gut homeostasis.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CCL25/TECK antibody 25285-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CCL25/TECK Polyclonal antibody (Cat# 25285-1-AP) has 1 total citation. It was cited in Nature Communications (IF 18.1) in a 2022 study titled "The immune landscape of human thymic epithelial tumors." The research field encompasses immunology and oncology, specifically investigating immune cell composition in thymic epithelial tumors using immunofluorescence (IF) on human samples.
| Species | Application | Title |
|---|---|---|
