Tested Applications
| Positive WB detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25303-1-AP targets TRIP12 in WB, IF, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag18112 Product name: Recombinant human TRIP12 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1691-2040 aa of BC114556 Sequence: QRADLGLWRGEEVTLSNPKGSQEGTKYIQNLQGLFALPFGRTAKPAHIAKVKMKFRFLGKLMAKAIMDFRLVDLPLGLPFYKWMLRQETSLTSHDLFDIDPVVARSVYHLEDIVRQKKRLEQDKSQTKESLQYALETLTMNGCSVEDLGLDFTLPGFPNIELKKGGKDIPVTIHNLEEYLRLVIFWALNEGVSRQFDSFRDGFESVFPLSHLQYFYPEELDQLLCGSKADTWDAKTLMECCRPDHGYTHDSRAVKFLFEILSSFDNEQQRLFLQFVTGSPRLPVGGFRSLNPPLTIVRKTFESTENPDDFLPSVMTCVNYLKLPDYSSIEIMREKLLIAAREGQQSFHLS Predict reactive species |
| Full Name | thyroid hormone receptor interactor 12 |
| Calculated Molecular Weight | 1992 aa, 220 kDa |
| Observed Molecular Weight | 190-220 kDa |
| GenBank Accession Number | BC114556 |
| Gene Symbol | TRIP12 |
| Gene ID (NCBI) | 9320 |
| RRID | AB_2880020 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14669 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TRIP12 antibody 25303-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TRIP12 antibody (Cat# 25303-1-AP) has 12 citations across journals including Nature Communications, Cell Discovery, Molecular Cell, Oncogene, Cell Reports, Clinical Epigenetics, Journal of Virology, and bioRxiv. Research fields span ubiquitin signaling, targeted protein degradation, oxidative stress response, cancer biology (bladder, lymphoma), HIV-1 replication, and epigenetic regulation. Applications are predominantly Western blot, with some IP/CoIP and IF.
| Species | Application | Title |
|---|---|---|
Nat Commun Targeted degradation of USP7 in solid cancer cells reveals distinct effects of deubiquitinase degraders and inhibitors. | ||
Cell Discov NUDT21 lactylation reprograms alternative polyadenylation to promote cuproptosis resistance | ||
Mol Cell TRIP12 promotes small-molecule-induced degradation through K29/K48-branched ubiquitin chains.
| ||
Mol Cell Deubiquitinases cleave ubiquitin-fused ribosomal proteins and physically counteract their targeting to the UFD pathway. |
Reviews
What customers say
The single review rates the product 5 stars. The reviewer, who tested it at 1:1000 dilution on U2OS, HeLa, and MDA-MB-231 cells for Western Blot and Immunofluorescence, states it is the only brand out of three that worked for their application. The only drawback noted is a nonspecific band appearing above the 250 kDa mark. Overall, the reviewer considers the antibody to perform very well.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Evra (Verified Customer) (09-04-2026) | We've tried three different brands, and this is the only one that works. My only gripe is that there appears to be a nonspecific band above the 250kDa mark. But other than that, it works really well.
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