Tested Applications
| Positive IHC detected in | human lung tissue, human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25309-1-AP targets PIH1D3 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15039 Product name: Recombinant human CXorf41 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-214 aa of BC033510 Sequence: MESENMDSENMKTENMESQNVDFESVSSVTALEALSKLLNPEEEDDSDYGQTNGLSTIGAMGPGNIGPPQIEELKVIPETSEENNEDIWNSEEIPEGAEYDDMWDVREIPEYEIIFRQQVGTEDIFLGLSKKDSSTGCCSELVAKIKLPNTNPSDIQIDIQETILDLRTPQKKLLITLPELVECTSAKAFYIPETETLEITMTMKRELDIANFF Predict reactive species |
| Full Name | chromosome X open reading frame 41 |
| Calculated Molecular Weight | 214 aa, 24 kDa |
| GenBank Accession Number | BC033510 |
| Gene Symbol | DNAAF6 |
| Gene ID (NCBI) | 139212 |
| RRID | AB_2880024 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NQM4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for PIH1D3 antibody 25309-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PIH1D3 Polyclonal antibody (Cat# 25309-1-AP) has 2 total citations. These appear in International Journal of Molecular Sciences (2025) and Frontiers in Cell and Developmental Biology (2023). The research fields include sperm flagella formation during spermiogenesis and ciliopathy involving dynein arm assembly in motile cilia. Applications cited include WB, IHC, and IF.
| Species | Application | Title |
|---|---|---|
Front Cell Dev Biol PIH1D3-knockout rats exhibit full ciliopathy features and dysfunctional pre-assembly and loading of dynein arms in motile cilia |
