CHERP Polyclonal antibody

CHERP Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25389-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

CHERP, DAN16, DAN26, ERPROT 213 21, SCAF6, SRA1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis of Jurkat using 25389-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25389-1-AP (CHERP Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 25389-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25389-1-AP (CHERP antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 25389-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25389-1-AP (CHERP Antibody) at dilution of 1:50 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 25389-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 25389-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 25389-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 25389-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 25389-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human kidney using 25389-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 25389-1-AP (CHERP antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 25389-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 25389-1-AP (CHERP Antibody) at dilution of 1:50 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, rat brain tissue, mouse brain tissue
Positive IHC detected in	mouse brain tissue, human kidney tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25389-1-AP targets CHERP in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag13428 Product name: Recombinant human CHERP protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 19-350 aa of BC021294 Sequence: EFYSYYKCKLALEQQQLICKQQTPELEPAATMPPLPQPPLAPAAPIPPAQGAPSMDELIQQSQWNLQQQEQHLLALRQEQVTAAVAHAVEQQMQKLLEETQLDMNEFDNLLQPIIDTCTKDAISAGKNWMFSNAKSPPHCELMAGHLRNRITADGAHFELRLHLIYLINDVLHHCQRKQARELLAALQKVVVPIYCTSFLAVEEDKQQKIARLLQLWEKNGYFDDSIIQQLQSPALGLGQYQATLINEYSSVVQPVQLAFQQQIQTLKTQHEEFVTSLAQQQQQQQQQQQQLQMPQMEAEVKATPPPPAPPPAPAPAPAIPPTTQPDDSKPP Predict reactive species
Full Name	calcium homeostasis endoplasmic reticulum protein
Calculated Molecular Weight	104 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	BC021294
Gene Symbol	CHERP
Gene ID (NCBI)	10523
RRID	AB_2880055
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IWX8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for CHERP antibody 25389-1-AP	Download protocol
WB protocol for CHERP antibody 25389-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The CHERP Polyclonal antibody (Cat# 25389-1-AP) has 1 total citation. It appears in iScience (Impact Factor 4.5), published in 2026. The cited study, "Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke," used this antibody for Western Blot in mouse samples. The research field is neuroscience, specifically focused on identifying diagnostic biomarkers and therapeutic targets for ischemic stroke through neuron-specific lineage analysis.

Species	Application	Title
mouse	WB	iScience Decoding neuron-specific lineage to identify diagnostic biomarkers and therapeutic targets for ischemic stroke. Authors - Xiaoya Wang KD Validated View Article