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KD/KO Validated

DKC1 Polyclonal antibody

DKC1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 25420-1-AP

Publications (4)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

CBF5, CBF5 homolog, DKC, DKC1, Dyskerin, NAP57, NOLA4, Nucleolar protein NAP57, snoRNP protein DKC1, XAP101

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHCIF/ICC

WB analysis of HepG2 using 25420-1-AP
WB result of DKC1 antibody (25420-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-DKC1 transfected HepG2 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 25420-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 25420-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of COLO 320 using 25420-1-AP
COLO 320 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 25420-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 25420-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 25420-1-AP (DKC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 25420-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 25420-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25420-1-AP (DKC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 25420-1-AP
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 25420-1-AP (DKC1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (4)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, COLO 320 cells, Jurkat cells, MCF-7 cells
Positive IHC detected in	human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2400
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25420-1-AP targets DKC1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag7992 Product name: Recombinant human DKC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 157-514 aa of BC009928 Sequence: VRLHNAIEGGTQLSRALETLTGALFQRPPLIAAVKRQLRVRTIYESKMIEYDPERRLGIFWVSCEAGTYIRTLCVHLGLLLGVGGQMQELRRVRSGVMSEKDHMVTMHDVLDAQWLYDNHKDESYLRRFVYPLEKLLTSHKRLVMKDSAVNAICYGAKIMLPGVLRYEDGIEVNQEIVVITTKGEAICMAIALMTTAVISTCDHGIVAKIKRVIMERDTYPRKWGLGPKASQKKLMIKQGLLDKHGKPTDSTPATWKQEYVDYSESAKKEVVAEVVKAPQVVAEAAKTAKRKRESESESDETPPAAPQLIKKEKKKSKKDKKAKAGLESGAEPGDGDSDTTKKKKKKKKAKEVELVSE Predict reactive species
Full Name	dyskeratosis congenita 1, dyskerin
Calculated Molecular Weight	58 kDa
Observed Molecular Weight	58 kDa
GenBank Accession Number	BC009928
Gene Symbol	DKC1
Gene ID (NCBI)	1736
RRID	AB_2880071
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O60832
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for DKC1 antibody 25420-1-AP	Download protocol
IHC protocol for DKC1 antibody 25420-1-AP	Download protocol
WB protocol for DKC1 antibody 25420-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The DKC1 Polyclonal antibody (Cat# 25420-1-AP) has 4 citations spanning Cell Reports, Frontiers in Cell and Developmental Biology, Stem Cells, and Journal of Cancer Research and Clinical Oncology. Research fields include DNA damage-mediated differentiation, telomerase biology in uterine endometrial carcinoma, mesenchymal stem cell self-renewal, and cuproptosis-related roles in esophageal carcinoma. All citations involve human samples, with applications covering WB, IHC, and IF.

Species	Application	Title
human	WB	Cell Rep Chromatin-associated orphan snoRNA regulates DNA damage-mediated differentiation via a non-canonical complex. Authors - Cai Han KD Validated View Article
human	IHC	Front Cell Dev Biol Comprehensive analyses of telomerase component DKC1 and its association with clinical, molecular and immune landscapes in uterine corpus endometrial carcinoma Authors - Chenxi Sun View Article
human	WB	Stem Cells H/ACA Box Small Nucleolar RNA 7A promotes the Self-Renewal of Human Umbilical Cord Mesenchymal Stem Cells. Authors - Yan Zhang View Article
human	WB,IHC,IF	J Cancer Res Clin Oncol Identifying and validating the roles of the cuproptosis-related gene DKC1 in cancer with a focus on esophageal carcinoma Authors - Daidi Zhang KD Validated View Article

Reviews

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1 verified customer reviews

What customers say

The single review for this product rates it 4 out of 5 stars. The reviewer reported that the antibody performed well in Western blot, using a 1:1000 dilution on human satellite muscle cells. Overall, the feedback is positive, confirming reliable performance for WB applications.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

Baptiste (Verified Customer) (09-05-2019)

This antibody works well in western blot