Tested Applications
| Positive WB detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human heart tissue, human skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25630-1-AP targets C9orf61 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22242 Product name: Recombinant human C9orf61 protein Source: e coli.-derived, PET30a Tag: 6*His Domain: 288-450 aa of BC113685 Sequence: PVLSCEAATQTERRLDLAAVTLRRGLRSRASRCRPRSLIDYKSYMDTKLLVARFLEQSSCTMTPDIHELVENIKSVLKSDEEHMEEAITSASFLEQIMAPLQPSTSRAHKLPSRRQPGLLHLQSCGDLHTFTPAGRPRAERRPRRVEAERPHSLIGVIRETVL Predict reactive species |
| Full Name | chromosome 9 open reading frame 61 |
| Calculated Molecular Weight | 450 aa, 50 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC113685 |
| Gene Symbol | C9orf61 |
| Gene ID (NCBI) | 9413 |
| RRID | AB_2880170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15884 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
C9orf61, also named as FAM189A2 and X123, is promoinently expressed in muscle. It has three isoforms with MW 45-50 kDa, 32-35 kDa and 27 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for C9orf61 antibody 25630-1-AP | Download protocol |
| WB protocol for C9orf61 antibody 25630-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The C9orf61 (FAM189A2) Polyclonal antibody, catalog number 25630-1-AP, has a total of 2 citations. These appear in the journals Journal of Translational Medicine (2025) and Tissue Cell (2024). The associated research fields focus on lung adenocarcinoma, examining C9orf61's role as a tumor suppressor, its activation of the Hippo signaling pathway, and its effects on glycolysis, proliferation, apoptosis, and tight junction proteins. Applications cited include WB, IHC, IF, and CoIP.
| Species | Application | Title |
|---|---|---|
J Transl Med FAM189A2 activates Hippo signaling pathway by abrogating WWP2-mediated LATS1 ubiquitination, to inhibit the glycolysis and proliferation processes of lung adenocarcinoma. | ||
Tissue Cell FAM189A2 plays a tumour suppressor role in lung adenocarcinoma by influencing cell apoptosis, CXCR4 expression and tight junction proteins |
Reviews
What customers say
The single review for this antibody (used in Western Blot at 1:1000 dilution on SH-SY5Y and HEK293T cells) was negative. The reviewer reported that the antibody could not reliably or specifically detect the protein of interest, producing many unspecific bands. Overall, the feedback suggests poor specificity and reliability for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Chrisovalantou (Verified Customer) (10-31-2024) | Antibody unfortunately could not reliably and specifically detect the protein of interest. Many unspecific bands.
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