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KD/KO Validated

KIAA1279 Polyclonal antibody

KIAA1279 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 25653-1-AP

Publications(4)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Synonyms

KBP, KIAA1279, KIF1 binding protein, TTC20

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 25653-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse kidney using 25653-1-AP
mouse kidney tissue were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HEK-293 using 25653-1-AP
HEK-293 cells were subjected to SDS PAGE followed by western blot with 25653-1-AP (KIAA1279 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 25653-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 25653-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse testis using 25653-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 25653-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 25653-1-AP (KIAA1279 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, mouse kidney tissue, NIH/3T3 cells
Positive IHC detected in	mouse kidney tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25653-1-AP targets KIAA1279 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag22234 Product name: Recombinant human KIAA1279 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 272-621 aa of BC012180 Sequence: CLSAANVIFGQTGKISATEDTPEAEGEVPELYHQRKGEIARCWIKYCLTLMQNAQLSMQDNIGELDLDKQSELRALRKKELDEEESIRKKAVQFGTGELCDAISAVEEKVSYLRPLDFEEARELFLLGQHYVFEAKEFFQIDGYVTDHIEVVQDHSALFKVLAFFETDMERRCKMHKRRIAMLEPLTVDLNPQYYLLVNRQIQFEIAHAYYDMMDLKVAIADRLRDPDSHIVKKINNLNKSALKYYQLFLDSLRDPNKVFPEHIGEDVLRPAMLAKFRVARLYGKIITADPKKELENLATSLEHYKFIVDYCEKHPEAAQEIEVELELSKEMVSLLPTKMERFRTKMALT Predict reactive species
Full Name	KIAA1279
Calculated Molecular Weight	621 aa, 72 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC012180
Gene Symbol	KIAA1279
Gene ID (NCBI)	26128
RRID	AB_2880178
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96EK5
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for KIAA1279 antibody 25653-1-AP	Download protocol
WB protocol for KIAA1279 antibody 25653-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
mouse	WB	Front Mol Neurosci Multiple Functions of KBP in Neural Development Underlie Brain Anomalies in Goldberg-Shprintzen Syndrome. Authors - Hsin-Yun Chang KD Validated View Article
mouse	WB, IF	Reproduction KIF1-binding protein interacts with KIF3A in haploid male germ cells. Authors - Mari S Lehti View Article
		bioRxiv A systems-level proteomic analysis identifies kinesin targets of KIFBP during neuronal development. Authors - Sarah-Catherine Paschall View Article
		Life Sci Alliance Axonal transport of CHMP2b is regulated by kinesin-binding protein and disrupted by CHMP2bintron5 Authors - Konner R Kirwan View Article