Tested Applications
| Positive WB detected in | A375 cells, HEK-293 cells, Neuro-2a cells, Y79 cells, HeLa cells |
| Positive IHC detected in | human colon tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A375 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:12000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25654-1-AP targets UACA in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22438 Product name: Recombinant human UACA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1075-1416 aa of BC113407 Sequence: LSQKYTEVKNVKEKLVEENAKQTSEILAVQNLLQKQHVPLEQVEALKKSLNGTIENLKEELKSMQRCYEKEQQTVTKLHQLLENQKNSSVPLAEHLQIKEAFEKEVGIIKASLREKEEESQNKMEEVSKLQSEVQNTKQALKKLETREVVDLSKYKATKSDLETQISSLNEKLANLNRKYEEVCEEVLHAKKKEISAKDEKELLHFSIEQEIKDQKERCDKSLTTITELQRRIQESAKQIEAKDNKITELLNDVERLKQALNGLSQLTYTSGNPTKRQSQLIDTLQHQVKSLEQQLADADRQHQEVIAIYRTHLLSAAQGHMDEDVQEALLQIIQMRQGLVC Predict reactive species |
| Full Name | uveal autoantigen with coiled-coil domains and ankyrin repeats |
| Calculated Molecular Weight | 1416 aa, 163 kDa |
| Observed Molecular Weight | 163 kDa |
| GenBank Accession Number | BC113407 |
| Gene Symbol | UACA |
| Gene ID (NCBI) | 55075 |
| RRID | AB_2880179 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BZF9 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for UACA antibody 25654-1-AP | Download protocol |
| IHC protocol for UACA antibody 25654-1-AP | Download protocol |
| WB protocol for UACA antibody 25654-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The UACA Polyclonal antibody (Cat# 25654-1-AP) has 6 total citations. These appear in Cell Reports (×2), Aging, Journal of Zhejiang University Science B, Journal of Oncology, and International Journal of Clinical and Experimental Pathology. Research fields span skeletal homeostasis and epigenetic regulation, basolateral exosome release, pancreatic cancer, PANoptosis in acute liver failure, glioma apoptosis via MAPK signaling, and hepatocellular carcinoma proliferation and senescence. Applications include WB and IF across human, canine, and mouse species.
| Species | Application | Title |
|---|---|---|
Cell Rep SUV39H1-dependent methyl-degron is a critical regulator of skeletal homeostasis. | ||
Cell Rep Rab39 and its effector UACA regulate basolateral exosome release from polarized epithelial cells. | ||
Aging (Albany NY) Transcriptome and proteome analysis of the antitumor activity of maslinic acid against pancreatic cancer cells | ||
J Zhejiang Univ Sci B Roles of PANoptosis and related genes in acute liver failure: neoteric insight from bioinformatics analysis and animal experiment verification | ||
J Oncol Maslinic Acid Inhibits the Growth of Malignant Gliomas by Inducing Apoptosis via MAPK Signaling. | ||
Int J Clin Exp Pathol Knockdown of UACA inhibitsproliferation and invasion and promotes senescence of hepatocellular carcinoma cells
|
