Tested Applications
| Positive WB detected in | HepG2 cells, HeLa cells |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26009-1-AP targets TRMT61B in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23307 Product name: Recombinant human TRMT61B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 129-477 aa of BC010365 Sequence: QSATEVEERHVSPSCSTSRERPFQAGELILAETGEGETKFKKLFRLNNFGLLNSNWGAVPFGKIVGKFPGQILRSSFGKQYMLRRPALEDYVVLMKRGTAITFPKDINMILSMMDINPGDTVLEAGSGSGGMSLFLSKAVGSQGRVISFEVRKDHHDLAKKNYKHWRDSWKLSHVEEWPDNVDFIHKDISGATEDIKSLTFDAVALDMLNPHVTLPVFYPHLKHGGVCAVYVVNITQVIELLDGIRTCELALSCEKISEVIVRDWLVCLAKQKNGILAQKVESKINTDVQLDSQEKIGVKGELFQEDDHEESHSDFPYGSFPYVARPVHWQPGHTAFLVKLRKVKPQLN Predict reactive species |
| Full Name | tRNA methyltransferase 61 homolog B (S. cerevisiae) |
| Observed Molecular Weight | 53 kDa |
| GenBank Accession Number | BC010365 |
| Gene Symbol | TRMT61B |
| Gene ID (NCBI) | 55006 |
| RRID | AB_2880333 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVS5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TRMT61B antibody 26009-1-AP | Download protocol |
| WB protocol for TRMT61B antibody 26009-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
TRMT61B Polyclonal antibody (Cat# 26009-1-AP) has 4 total citations. These appear in Nature Communications, Cell Discovery, Cancer Letters, and Cell Molecular Life Sciences. The associated research fields include RNA modification, mitochondrial tRNA biology, ciliogenesis, tumor growth and metastasis in breast cancer, and mitoribosomal biogenesis. All cited applications are Western blot (WB) in human samples.
| Species | Application | Title |
|---|---|---|
Nat Commun RNA modification landscape of the human mitochondrial tRNALys regulates protein synthesis. | ||
Cancer Lett YTHDF3 Drives Tumor Growth and Metastasis by Recruiting eIF4B to Promote Notch2 Translation in Breast Cancer | ||
Cell Mol Life Sci GTPBP8 is required for mitoribosomal biogenesis and mitochondrial translation |
