Tested Applications
| Positive WB detected in | SH-SY5Y cells, HeLa cells, K-562 cells |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26078-1-AP targets MAP9 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag23446 Product name: Recombinant human MAP9 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-349 aa of BC146864 Sequence: MSDEVFSTTLAYTKSPKVTKRTTFQDELVRAITARSARQRSSEYSDDFDSDEIVSLGDFSDTSADENSVNKKMNDFHISDDEEKNPSKLLFLKTNKSNGNITKDEPVCAIKNEEEMAPDGCEDIVVKSFSESQNKDEEFEKDKIKMKPKPRILSIKSTSSAENNSLDTDDHFKPSPRPRSMLKKKSHMEEKDGLEDKETALSEELELHSAPSSLPTPNGIQLEAEKKAFSENLDPEDSCLTSLASSSLKQILGDSFSPGSEGNASGKDPNEEITENHNSLKSDENKENSFSADHVTTAVEKSKESQVTADDLEEEKAKAELIMDDDRTVDPLLSKSQSILISTSATASS Predict reactive species |
| Full Name | microtubule-associated protein 9 |
| Observed Molecular Weight | 38-42 kDa |
| GenBank Accession Number | BC146864 |
| Gene Symbol | MAP9 |
| Gene ID (NCBI) | 79884 |
| RRID | AB_2880366 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q49MG5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MAP9 antibody 26078-1-AP | Download protocol |
| IHC protocol for MAP9 antibody 26078-1-AP | Download protocol |
| WB protocol for MAP9 antibody 26078-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAP9 Polyclonal antibody (Catalog No. 26078-1-AP) has 4 total citations. These appear in high-impact journals including Developmental Cell, EMBO Journal, Frontiers in Cellular Neuroscience, and Science. The associated research fields focus on cilia biology, covering axonemal microtubule structure, photoreceptor cilia, ciliary transition zones, and related disorders such as cone-rod dystrophy and primary ciliary dyskinesia. Applications cited include IHC and IF.
| Species | Application | Title |
|---|---|---|
EMBO J Glutamylation imbalance impairs the molecular architecture of the photoreceptor cilium | ||
Front Cell Neurosci Molecular characterization of MAP9 in the photoreceptor sensory cilia as a modifier in canine RPGRIP1-associated cone-rod dystrophy | ||
Science In situ structure of the human ciliary transition zone links linker defects to primary ciliary dyskinesia. |
