ZNF189 Polyclonal antibody

ZNF189 Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 26169-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, ELISA

Synonyms

Zinc finger protein 189

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIF/ICC

WB analysis using 26169-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26169-1-AP (ZNF189 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 26169-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZNF189 antibody (26169-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, THP-1 cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26169-1-AP targets ZNF189 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag23264 Product name: Recombinant human ZNF189 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-150 aa of BC126120 Sequence: MASPSPPPESKEEWDYLDPAQRSLYKDVMMENYGNLVSLDVLNRDKDEEPTVKQEIEEIEEEVEPQGVIVTRIKSEIDQDPMGRETFELVGRLDKQRGIFLWEIPRESLTQEQRMFRENTNIIRKRPNSEEKCHKCEECGKGFVRKAHFI Predict reactive species
Full Name	zinc finger protein 189
Observed Molecular Weight	68 kDa
GenBank Accession Number	BC126120
Gene Symbol	ZNF189
Gene ID (NCBI)	7743
RRID	AB_3742172
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O75820
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for ZNF189 antibody 26169-1-AP	Download protocol
WB protocol for ZNF189 antibody 26169-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The ZNF189 Polyclonal antibody (Cat# 26169-1-AP) has 1 total citation. It appears in FEBS Journal (Impact Factor 4.2), in a 2023 study by Xiao Li et al. titled "Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice." The research field is retinal biology and photoreceptor degeneration, with the antibody applied via Western Blot in mouse samples.