Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | rat brain tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26426-1-AP targets KCNH1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16647 Product name: Recombinant human KCNH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 686-850 aa of BC113709 Sequence: VKREEEERMKRKNEAPLILPPDHPVRRLFQRFRQQKEARLAAERGGRDLDDLDVEKGNVLTEHASANHSLVKASVVTVRESPATPVSFQAASTSGVPDHAKLQAPGSECLGPKGGGGDCAKRKSWARFKDACGKSEDWNKVSKAESMETLPERTKASGEATLKKT Predict reactive species |
| Full Name | potassium voltage-gated channel, subfamily H (eag-related), member 1 |
| Calculated Molecular Weight | 989 aa, 111 kDa |
| Observed Molecular Weight | 111 kDa |
| GenBank Accession Number | BC113709 |
| Gene Symbol | KCNH1 |
| Gene ID (NCBI) | 3756 |
| RRID | AB_3742187 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95259 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Potassium voltage-gated channel subfamily H member 1 (KCNH1, also known as Kv10.1 and EAG) a member of the EAG (ether-à-go-go) family, which is involved in intracellular signaling, cell proliferation, and tumorigenesis (PMID: 35639255). KCNH1 is mainly expressed in the adult central nervous system. It also plays a role in controlling K+ fux that regulates resting membrane potential in excitable and non-excitable cells and is activated at the onset of myoblast differentiation (PMID: 29333676; 27029528; 9738473).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KCNH1 antibody 26426-1-AP | Download protocol |
| IP protocol for KCNH1 antibody 26426-1-AP | Download protocol |
| WB protocol for KCNH1 antibody 26426-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The KCNH1 Polyclonal antibody (Cat# 26426-1-AP) has 1 total citation. It was published in Frontiers in Bioscience (Landmark Edition) in 2025. The study investigated co-highly expressed SLC17A9 and KCNH1 as potential prognostic biomarkers and therapeutic targets in clear cell renal cell carcinoma, placing this citation within the fields of cancer biology and renal cell carcinoma research. The antibody was applied in Western blot and immunofluorescence using human samples.
| Species | Application | Title |
|---|---|---|
Front Biosci (Landmark Ed) Co-Highly Expressed SLC17A9 and KCNH1 as Potential Prognostic Biomarkers and Therapeutic Targets in Clear Cell Renal Cell Carcinoma
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