Tested Applications
| Positive WB detected in | Daudi cells, HeLa cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26591-1-AP targets MTHFR in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14970 Product name: Recombinant human MTHFR protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 307-656 aa of BC053509 Sequence: QELLASGLVPGLHFYTLNREMATTEVLKRLGMWTEDPRRPLPWALSAHPKRREEDVRPIFWASRPKSYIYRTQEWDEFPNGRWGNSSSPAFGELKDYYLFYLKSKSPKEELLKMWGEELTSEASVFEVFVLYLSGEPNRNGHKVTCLPWNDEPLAAETSLLKEELLRVNRQGILTINSQPNINGKPSSDPIVGWGPSGGYVFQKAYLEFFTSRETAEALLQVLKKYELRVNYHLVNVKGENITNAPELQPNAVTWGIFPGREIIQPTVVDPVSFMFWKDEAFALWIEQWGKLYEEESPSRTIIQYIHDNYFLVNLVDNDFPLDNCLWQVVEDTLELLNRPTQNARETEAP Predict reactive species |
| Full Name | 5,10-methylenetetrahydrofolate reductase (NADPH) |
| Calculated Molecular Weight | 656 aa, 75 kDa |
| Observed Molecular Weight | 75-78 kDa |
| GenBank Accession Number | BC053509 |
| Gene Symbol | MTHFR |
| Gene ID (NCBI) | 4524 |
| RRID | AB_3085887 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42898 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for MTHFR antibody 26591-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MTHFR polyclonal antibody (Cat# 26591-1-AP) has 2 total citations. These appear in Frontiers in Pharmacology and Evidence-Based Complementary and Alternative Medicine. The research fields span hepatology (non-alcoholic steatohepatitis and homocysteine-pathway regulation) and cardiology (endoplasmic reticulum stress, unfolded protein response, and ventricular remodeling post-myocardial infarction). Both studies utilized the antibody for Western blot in rat models.
| Species | Application | Title |
|---|---|---|
Front Pharmacol Effect of gentiopicroside on endogenous formaldehyde homocysteine-pathway related proteins in rats with non-alcoholic steatohepatitis. | ||
Evid Based Complement Alternat Med Wenxin Granules Regulate Endoplasmic Reticulum Stress Unfolded Protein Response and Improve Ventricular Remodeling on Rats with Myocardial Infarction |
