WSTF Polyclonal antibody

WSTF Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 26641-1-AP

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

BAZ1B, EC:2.7.10.2, hWALp2, Tyrosine protein kinase BAZ1B, Tyrosine-protein kinase BAZ1B

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 26641-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 26641-1-AP (WSTF antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 26641-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26641-1-AP (WSTF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat ovary using 26641-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 26641-1-AP (WSTF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human ovary cancer using 26641-1-AP
Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 26641-1-AP (WSTF antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 26641-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 26641-1-AP (WSTF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat brain using 26641-1-AP
Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 26641-1-AP (WSTF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat ovary using 26641-1-AP
Immunohistochemical analysis of paraffin-embedded rat ovary tissue slide using 26641-1-AP (WSTF antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 26641-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using WSTF antibody (26641-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	SH-SY5Y cells, HEK-293 cells, HeLa cells, HepG2 cells
Positive IHC detected in	rat ovary tissue, human ovary cancer tissue, mouse brain tissue, rat brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

26641-1-AP targets WSTF in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag24403 Product name: Recombinant human BAZ1B protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1144-1483 aa of BC136520 Sequence: KVASALEKWKTAIREAQTFSRMHVLLGMLDACIKWDMSAENARCKVCRKKGEDDKLILCDECNKAFHLFCLRPALYEVPDGEWQCPACQPATARRNSRGRNYTEESASEDSEDDESDEEEEEEEEEEEEEDYEVAGLRLRPRKTIRGKHSVIPPAARSGRRPGKKPHSTRRSQPKAPPVDDAEVDELVLQTKRSSRRQSLELQKCEEILHKIVKYRFSWPFREPVTRDEAEDYYDVITHPMDFQTVQNKCSCGSYRSVQEFLTDMKQVFTNAEVYNCRGSHVLSCMVKTEQCLVALLHKHLPGHPYVRRKRKKFPDRLAEDEGDSEPEAVGQSRGRRQKK Predict reactive species
Full Name	bromodomain adjacent to zinc finger domain, 1B
Calculated Molecular Weight	1483 aa, 171 kDa
Observed Molecular Weight	170 kDa
GenBank Accession Number	BC136520
Gene Symbol	WSTF/BAZ1B
Gene ID (NCBI)	9031
RRID	AB_3742196
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UIG0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for WSTF antibody 26641-1-AP	Download protocol
IHC protocol for WSTF antibody 26641-1-AP	Download protocol
WB protocol for WSTF antibody 26641-1-AP	Download protocol