Tested Applications
| Positive WB detected in | K-562 cells, mouse brain tissue |
| Positive IP detected in | U2OS cells |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26781-1-AP targets VGF in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag24820 Product name: Recombinant human VGF protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 81-280 aa of BC063835 Sequence: ALAAVLLQALDRPASPPAPSGSQQGPEEEAAEALLTETVRSQTHSLPAPESPEPAAPPRPQTPENGPEASDPSEELEALASLLQELRDFSPSSAKRQQETAAAETETRTHTLTRVNLESPGPERVWRASWGEFQARVPERAPLPPPAPSQFQARMPDSGPLPETHKFGEGVSSPKTHLGEALAPLSKAYQGVAAPFPKAR Predict reactive species |
| Full Name | VGF nerve growth factor inducible |
| Calculated Molecular Weight | 615 aa, 67 kDa |
| Observed Molecular Weight | 67 kDa, 90-100 kDa |
| GenBank Accession Number | BC063835 |
| Gene Symbol | VGF |
| Gene ID (NCBI) | 7425 |
| RRID | AB_2880632 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15240 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VGF encodes a neuropeptide precursor with specific expression in a subpopulation of neuroendocrine cells, and it is upregulated by nerve growth factor. The expression of VGF is developmentally regulated in different areas of the brain (PubMed: 18406753). Exercise upregulated expression of VGF in mouse hippocampus, and a synthetic Vgf-derived peptide produced an antidepressant response in mice (PubMed: 18059283). VGF can be detected the precursor form of 90-100 kDa before proteolytic cleavage (PMID:24934694, PMID:10433233).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VGF antibody 26781-1-AP | Download protocol |
| WB protocol for VGF antibody 26781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VGF Polyclonal antibody (catalog number 26781-1-AP) has 3 total citations. These appear in the Journal of Experimental & Clinical Cancer Research, Clinical Epigenetics, and Molecular Biology of the Cell. The associated research fields include pancreatic cancer (exosomal circRNA/miRNA axis), lung adenocarcinoma (m6A methylation and histone modification), and neuronal biology (microtubule dynamics and kinesin-4 motor motility in dendrites).
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Hedgehog-Gli1-derived exosomal circ-0011536 mediates peripheral neural remodeling in pancreatic cancer by modulating the miR-451a/VGF axis | ||
Clin Epigenetics METTL3 exerts synergistic effects on m6A methylation and histone modification to regulate the function of VGF in lung adenocarcinoma | ||
Mol Biol Cell Microtubule dynamics influence the retrograde biased motility of kinesin-4 motor teams in neuronal dendrites. |
Reviews
What customers say
The single review for this antibody is a 5-star rating for Western Blot. The reviewer used a 1:1000 dilution on HeLa cell lysate and an IVT expression system, reporting that the antibody performed well in detecting both GST-tagged VGF in HeLa cell lysates and HRV 3C protease-cleaved VGF following IVT reaction and GST purification. Overall, the feedback highlights reliable specificity and sensitivity across different sample preparations.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
James (Verified Customer) (09-15-2020) | Antibody works well, it is able to detect both GST-tagged VGF expressed in HeLa cell lysates, and HRV 3C protease cleaved VGF after IVT reaction and GST purification.
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