Tested Applications
| Positive WB detected in | K-562 cells, MOLT-4 cells, Raji cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26923-1-AP targets VPS35L in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25620 Product name: Recombinant human C16orf62 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 579-702 aa of BC058845 Sequence: ESVRVEVCKCIMDAFIKHQQEPTKDPVILNALLHVCKTMHDSVNALTLEDEKRMLSYLINGFIKMVSFGRDFEQQLSFYVESRSMFCNLEPVLVQLIHSVNRLAMETRKVMKGNHSRKTAAFVR Predict reactive species |
| Full Name | chromosome 16 open reading frame 62 |
| Observed Molecular Weight | 98 kDa |
| GenBank Accession Number | BC058845 |
| Gene Symbol | C16orf62 |
| Gene ID (NCBI) | 57020 |
| RRID | AB_3669574 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q7Z3J2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VPS35L, also known as EC97, contains 5 N-glycosylation sites, 3 N-myristoylation sites, and numerous phosphorylation sites. Northern blot analysis detected a 3.4-kb EC97 transcript in all human tissues tested except adipose. RT-PCR analysis showed that EC97 expression was upregulated in 60% of human esophageal squamous cell carcinoma (ESCC) tissues and downregulated in 35% of ESCC tissues tested compared with controls. VPS35L is Involved in Golgi to plasma membrane transport and endocytic recycling.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS35L antibody 26923-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for this product is a 5-star rating. The reviewer used it for Western Blot at a 1:500 dilution and described it simply as "good for WB." Overall, the feedback is positive, indicating the antibody performed well in its intended application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Ahmad (Verified Customer) (09-05-2026) | good for wb
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