Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, MCF-7 cells, Jurkat cells |
| Positive IP detected in | K-562 cells |
| Positive IHC detected in | mouse brain tissue, human breast cancer tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
26971-1-AP targets DNMT3B in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25117 Product name: Recombinant human DNMT3B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-100 aa of NM_001207055 Sequence: MKGDTRHLNGEEDAGGREDSILVNGACSDQSSDSPPILEAIRTPEIRGRRSSSRLSKREVSSLLSYTQDLTGDGDGEDGDGSDTPVMPKLFRETRTRSES Predict reactive species |
| Full Name | DNA (cytosine-5-)-methyltransferase 3 beta |
| Calculated Molecular Weight | 95 kDa |
| Observed Molecular Weight | 85-96 kDa |
| GenBank Accession Number | NM_001207055 |
| Gene Symbol | DNMT3B |
| Gene ID (NCBI) | 1789 |
| RRID | AB_2880705 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBC3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
DNMT3B is a DNA-(cytosine C5)-methyltransferase that is responsible for establishing the methylation patterns in cooperation with DNMT3A through the de novo methylation pathway. It is essential for the establishment of DNA methylation patterns during development. DNMT3B contains a C-terminal catalytic domain and an N-terminal regulatory domain, which are involved in targeting chromatin to regulate its function (PMID: 31547729). DNMT3B has 8 isoforms with the molecular mass of 80-96 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DNMT3B antibody 26971-1-AP | Download protocol |
| IP protocol for DNMT3B antibody 26971-1-AP | Download protocol |
| WB protocol for DNMT3B antibody 26971-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DNMT3B Polyclonal antibody (Cat# 26971-1-AP) has 61 citations. These appear in journals including Nature Communications, Advanced Science, Cell Reports, Biomaterials, Brain, Free Radical Biology and Medicine, Journal of Biological Chemistry, Redox Biology, and Theranostics, among others. Research fields span epigenetics and DNA methylation, cancer (lung, colorectal, gastric, breast, prostate, leukemia), fibrosis, cardiovascular disease, neurology, renal disease, and immunology, with applications mainly in Western blot, IHC, IF, and ChIP.
| Species | Application | Title |
|---|---|---|
Protein Cell The chemical reprogramming of unipotent adult germ cells towards authentic pluripotency and de novo establishment of imprinting | ||
Nat Commun Lactate mitochondrial oxidation drives stemness potential in metastatic breast cancer. | ||
J Adv Res UHRF1-mediated DNA 5-mC modification drives super-enhancer redistribution and impedes osteogenesis via TGM2-regulated autophagic flux in senile osteoporosis. | ||
Redox Biol ROS promote hyper-methylation of NDRG2 promoters in a DNMTS-dependent manner: Contributes to the progression of renal fibrosis | ||
Theranostics Targeting CPS1 attenuates lung cancer metastasis by regulating EMT through an epigenetic mechanism. |
