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What's the best dilution for KCNK4 in ?
Tested Applications
| Positive WB detected in | mouse eye tissue, rat eye tissue |
| Positive IHC detected in | human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27113-1-AP targets KCNK4 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25931 Product name: Recombinant human KCNK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 261-393 aa of BC033577 Sequence: SRRTRAEMGGLTAQAASWTGTVTARVTQRAGPAAPPPEKEQPLLPPPPCPAQPLGRPRSPSPPEKAQPPSPPTASALDYPSENLAFIDESSDTQSERGCPLPRAPRGRRRPNPPRKPVRPRGPGRPRDKGVPV Predict reactive species |
| Full Name | potassium channel, subfamily K, member 4 |
| Calculated Molecular Weight | 43 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | BC033577 |
| Gene Symbol | KCNK4 |
| Gene ID (NCBI) | 50801 |
| RRID | AB_2880761 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NYG8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for KCNK4 antibody 27113-1-AP | Download protocol |
| WB protocol for KCNK4 antibody 27113-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
KCNK4 Polyclonal antibody (27113-1-AP)
- Antibody name: KCNK4 Polyclonal antibody
- Catalog number: 27113-1-AP
- Total citation count: 1
- Journal: Functional & Integrative Genomics (Funct Integr Genomics)
- Research field: Oncology — specifically, the citation investigates lncRNA SLC25A21-AS1's role in enhancing chemosensitivity and inhibiting ovarian cancer progression by upregulating KCNK4 expression (validated by Western blot in human samples).
This single published citation situates the antibody within the ovarian cancer/chemoresistance research field.
| Species | Application | Title |
|---|---|---|
Funct Integr Genomics LncRNA SLC25A21-AS1 increases the chemosensitivity and inhibits the progression of ovarian cancer by upregulating the expression of KCNK4 |
