ADAL Polyclonal antibody

ADAL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27369-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

ADAL, adenosine deaminase like

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 27369-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27369-1-AP (ADAL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of LNCaP using 27369-1-AP
LNCaP cells were subjected to SDS PAGE followed by western blot with 27369-1-AP (ADAL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 27369-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human heart using 27369-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 27369-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 27369-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27369-1-AP (ADAL Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	Jurkat cells, LNCaP cells, K-562 cells
Positive IHC detected in	human heart tissue, human breast cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27369-1-AP targets ADAL in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag25949 Product name: Recombinant human ADAL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 31-200 aa of BC075857 Sequence: ISSHTMKKLIAQKPDLKIHDQMTVIDKGKKRTLEECFQMFQTIHQLTSSPEDILMVTKDVIKEFADDGVKYLELRSTPRRENATGMTKKTYVESILEGIKQSKQENLDIDVRYLIAVDRRGGPLVAKETVKLAEEFFLSTEGTVLGLDLSGDPTVGQAKDFLEPLLEAKK Predict reactive species
Full Name	adenosine deaminase-like
Calculated Molecular Weight	267 aa, 30 kDa
Observed Molecular Weight	38 kDa
GenBank Accession Number	BC075857
Gene Symbol	ADAL
Gene ID (NCBI)	161823
RRID	AB_2880855
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q6DHV7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for ADAL antibody 27369-1-AP	Download protocol
WB protocol for ADAL antibody 27369-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
		Cell Adenosine kinase and ADAL coordinate detoxification of modified adenosines to safeguard metabolism Authors - Akiko Ogawa View Article
mouse	WB	EMBO J Aberrant DNA N6 -methyladenine incorporation via adenylate kinase 1 is suppressed by ADAL deaminase-dependent 2'-deoxynucleotide pool sanitation Authors - Shaokun Chen View Article