Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27701-1-AP targets ANKRD17 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag26767 Product name: Recombinant human ANKRD17 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1553-1684 aa of NM_001286771 Sequence: MGSHGKRNNTITTTSSKRKNRKNKITPENVQIIFDDPLPISYSQPEKVNGESKSSSTSESGDSDNMRISSCSDESSNSNSSRKSDNHSPAVVTTTVSSKKQPSVLVTFPKEERKSVSGKASIKLSETISEGTS Predict reactive species |
| Full Name | ankyrin repeat domain 17 |
| Calculated Molecular Weight | 274 kDa |
| Observed Molecular Weight | 270-300 kDa |
| GenBank Accession Number | NM_001286771 |
| Gene Symbol | ANKRD17 |
| Gene ID (NCBI) | 26057 |
| RRID | AB_3085987 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75179 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ANKRD17 belongs to the family of ankyrin repeat-containing proteins, and contains two distinct arrays of ankyrin repeats in its amino-terminal region. It also contains a nuclear export signal, nuclear localization signal, and a cyclin-binding RXL motif. Localization of this protein to the nucleus has been shown experimentally, and interactions between this protein and cyclin-dependent kinase 2 have been observed.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for ANKRD17 antibody 27701-1-AP | Download protocol |
| WB protocol for ANKRD17 antibody 27701-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The ANKRD17 Polyclonal antibody (Cat# 27701-1-AP) has 1 total citation. It appears in the Journal of Neurodevelopmental Disorders (2025), where it was used by Western blot to study novel ANKRD17 variants implicated in synaptic and mitochondrial disruptions associated with intellectual disability and autism spectrum disorder. The research field encompasses neurodevelopmental genetics, synaptic biology, and mitochondrial function in the context of neurodevelopmental disorders.
| Species | Application | Title |
|---|---|---|
J Neurodev Disord Novel ANKRD17 variants implicate synaptic and mitochondrial disruptions in intellectual disability and autism spectrum disorder |
