Tested Applications
| Positive WB detected in | HEK-293T cells, HL-60 cells, Jurkat cells, K-562 cells, THP-1 cells |
| Positive IHC detected in | mouse testis tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse small intestine tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27879-1-AP targets NEU3 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27259 Product name: Recombinant human NEU3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 193-326 aa of NM_006656.5 Sequence: MPCKTRPHSLMIYSDDLGVTWHHGRLIRPMVTVECEVAEVTGRAGHPVLYCSARTPNRCRAEALSTDHGEGFQRLALSRQLCEPPHGCQGSVVSFRPLEIPHRCQDSSSKDAPTIQQSSPGSSLRLEEEAGTPSE Predict reactive species |
| Full Name | sialidase 3 (membrane sialidase) |
| Calculated Molecular Weight | 48 kDa |
| Observed Molecular Weight | 48, 52 kDa |
| GenBank Accession Number | NM_006656.5 |
| Gene Symbol | NEU3 |
| Gene ID (NCBI) | 10825 |
| RRID | AB_2881002 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UQ49 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for NEU3 antibody 27879-1-AP | Download protocol |
| IHC protocol for NEU3 antibody 27879-1-AP | Download protocol |
| WB protocol for NEU3 antibody 27879-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The NEU3 Polyclonal antibody (Cat# 27879-1-AP) has 7 citations spanning journals including J Control Release, PLoS Biol, JCI Insight, Food Funct, Cells, PLoS One, and Exp Lung Res. Research fields covered include liver cancer, breast cancer, pulmonary fibrosis, colorectal cancer, liver inflammation, and lung sialidase biology. Applications cited are WB, IHC, and IF in human and mouse models.
| Species | Application | Title |
|---|---|---|
J Control Release Tumor-specific glyco-detonators: A neuraminidase-3-gated programmable DNA Nanoarchitectonics for liver cancer-exclusive chemotherapy. | ||
PLoS Biol The mTORC2 subunit RICTOR drives breast cancer progression by promoting ganglioside biosynthesis through transcriptional and epigenetic mechanisms. | ||
JCI Insight The mRNA binding protein DDX3 mediates TGF-β1 upregulation of translation and promotes pulmonary fibrosis | ||
Food Funct Polyphenol from millet bran increases the sensitivity of colorectal cancer cells to oxaliplatin by blocking the ganglioside GM3 catabolism.
| ||
Cells Latency-Associated Peptide Rapidly Upregulates Neuraminidase 3 in a Profibrotic Translation-Based Positive Feedback Loop. | ||
Reviews
What customers say
The single review reports a negative experience with this antibody for Western Blot on mouse liver tissue at a 1:2000 dilution. The user noted that while the datasheet displays a clear Neu3 band, they observed multiple bands instead and concluded the antibody did not work for their application. They speculated the discrepancy might be related to a different antibody lot. Overall, the reviewer rated the product 1 out of 5 stars.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Prem (Verified Customer) (10-21-2022) | In the data sheet, the clear band of Neu3 was shown but in my case there are multiple bands. I dont know it might be because of different lot of antibody. it did not work for my western blot.
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