Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-Fro detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
27929-1-AP targets GABRA5 in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27376 Product name: Recombinant human GABRA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 36-222 aa of BC113422 Sequence: SSVKDETNDNITIFTRILDGLLDGYDNRLRPGLGERITQVRTDIYVTSFGPVSDTEMEYTIDVFFRQSWKDERLRFKGPMQRLPLNNLLASKIWTPDTFFHNGKKSIAHNMTTPNKLLRLEDDGTLLYTMRLTISAECPMQLEDFPMDAHACPLKFGSYAYPNSEVVYVWTNGSTKSVVVAEDGSRL Predict reactive species |
| Full Name | gamma-aminobutyric acid (GABA) A receptor, alpha 5 |
| Calculated Molecular Weight | 462 aa, 52 kDa |
| Observed Molecular Weight | 52 kDa |
| GenBank Accession Number | BC113422 |
| Gene Symbol | GABRA5 |
| Gene ID (NCBI) | 2558 |
| RRID | AB_2923580 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P31644 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for GABRA5 antibody 27929-1-AP | Download protocol |
| IHC protocol for GABRA5 antibody 27929-1-AP | Download protocol |
| WB protocol for GABRA5 antibody 27929-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The GABRA5 Polyclonal antibody (Cat# 27929-1-AP) has 2 citations. These appear in the British Journal of Anaesthesia and Acta Neuropathologica. The associated research fields include anesthesiology (sevoflurane-induced cognitive impairment via GABA(A) receptor trafficking in aged mice) and neurodegeneration (neurodegenerative phenotype in Down syndrome pyramidal neurons).
| Species | Application | Title |
|---|---|---|
Br J Anaesth Sevoflurane alters α5β3GABA(A) receptor trafficking via calcium/calmodulin-dependent protein kinase II-dependent β3 subunit phosphorylation to produce cognitive impairment in aged mice. | ||
Acta Neuropathol Analysis of microisolated frontal cortex excitatory layer III and V pyramidal neurons reveals a neurodegenerative phenotype in individuals with Down syndrome. |
