GABRA5 Polyclonal antibody

GABRA5 Polyclonal Antibody for WB, IHC, IF-Fro, ELISA

Cat No. 27929-1-AP

Publications (2)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-Fro, ELISA

Synonyms

GABA A receptor alpha 5, GABA(A) receptor subunit alpha-5, GABAA receptor alpha 5, GABAAR subunit alpha-5, Gamma-aminobutyric acid receptor subunit alpha-5

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF-Fro

WB analysis using 27929-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27929-1-AP (GABRA5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 27929-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27929-1-AP (GABRA5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 27929-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 27929-1-AP (GABRA5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 27929-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using GABRA5 antibody (27929-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IHC detected in	mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-Fro detected in	mouse brain tissue

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27929-1-AP targets GABRA5 in WB, IHC, IF-Fro, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag27376 Product name: Recombinant human GABRA5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 36-222 aa of BC113422 Sequence: SSVKDETNDNITIFTRILDGLLDGYDNRLRPGLGERITQVRTDIYVTSFGPVSDTEMEYTIDVFFRQSWKDERLRFKGPMQRLPLNNLLASKIWTPDTFFHNGKKSIAHNMTTPNKLLRLEDDGTLLYTMRLTISAECPMQLEDFPMDAHACPLKFGSYAYPNSEVVYVWTNGSTKSVVVAEDGSRL Predict reactive species
Full Name	gamma-aminobutyric acid (GABA) A receptor, alpha 5
Calculated Molecular Weight	462 aa, 52 kDa
Observed Molecular Weight	52 kDa
GenBank Accession Number	BC113422
Gene Symbol	GABRA5
Gene ID (NCBI)	2558
RRID	AB_2923580
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P31644
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for GABRA5 antibody 27929-1-AP	Download protocol
IHC protocol for GABRA5 antibody 27929-1-AP	Download protocol
WB protocol for GABRA5 antibody 27929-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

GABRA5 Polyclonal antibody (catalog number 27929-1-AP) has a total of 2 citations, published in British Journal of Anaesthesia and Acta Neuropathologica. The research fields associated with these citations include anesthesia-induced cognitive impairment (sevoflurane effects on α5β3GABA(A) receptor trafficking and phosphorylation) and neurodegenerative phenotypes in Down syndrome (frontal cortex pyramidal neurons).

Species	Application	Title
human	WB	Acta Neuropathol Analysis of microisolated frontal cortex excitatory layer III and V pyramidal neurons reveals a neurodegenerative phenotype in individuals with Down syndrome. Authors - Melissa J. Alldred View Article
		Br J Anaesth Sevoflurane alters α5β3GABA(A) receptor trafficking via calcium/calmodulin-dependent protein kinase II-dependent β3 subunit phosphorylation to produce cognitive impairment in aged mice. Authors - Tiantian Wan View Article