EIF2AK4 Polyclonal antibody

EIF2AK4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 27944-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

EC:2.7.11.1, eIF-2-alpha kinase GCN2, Eukaryotic translation initiation factor 2-alpha kinase 4, GCN2, GCN2 like protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIHC

WB analysis using 27944-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 27944-1-AP (EIF2AK4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 27944-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27944-1-AP (EIF2AK4 antibody) at dilution of 1:100 (under 40x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human small intestine using 27944-1-AP
Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 27944-1-AP (EIF2AK4 antibody) at dilution of 1:100 (under 10x lens).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	HepG2 cells, TT cells
Positive IHC detected in	human small intestine tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

27944-1-AP targets EIF2AK4 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag27617 Product name: Recombinant human EIF2AK4 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 875-1080 aa of BC009350 Sequence: AFSADSKQDDQTGDLIKSDPSGHLTGMVGTALYVSPEVQGSTKSAYNQKVDLFSLGIIFFEMSYHPMVTASERIFVLNQLRDPTSPKFPEDFDDGEHAKQKSVISWLLNHDPAKRPTATELLKSELLPPPQMEESELHEVLHHTLTNVDGKAYRTMMAQIFSQRISPAIDYTYDSDILKGNFSIRTAKMQQHVCETIIRIFKRHGA Predict reactive species
Full Name	eukaryotic translation initiation factor 2 alpha kinase 4
Calculated Molecular Weight	187 kDa
Observed Molecular Weight	~200 kDa
GenBank Accession Number	BC009350
Gene Symbol	EIF2AK4
Gene ID (NCBI)	440275
RRID	AB_3669630
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9P2K8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IHC protocol for EIF2AK4 antibody 27944-1-AP	Download protocol
WB protocol for EIF2AK4 antibody 27944-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
		Sci Rep Leucyl tRNA synthetase ameliorates cholestatic liver injury by inhibiting integrated stress response in mice. Authors - Pengfei Yan View Article
human	WB	bioRxiv A dietary pan-amino acid dropout screen in vivo reveals a critical role for histidine in T-ALL. Authors - Komal Mandleywala View Article