Tested Applications
| Positive WB detected in | A431 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28005-1-AP targets Tissue Factor/CD142 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27553 Product name: Recombinant human F3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 113-251 aa of BC011029 Sequence: GNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTKVNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRTVNRKSTDSPVECMGQEKGEFRE Predict reactive species |
| Full Name | coagulation factor III (thromboplastin, tissue factor) |
| Calculated Molecular Weight | 295 aa, 33 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC011029 |
| Gene Symbol | F3 |
| Gene ID (NCBI) | 2152 |
| RRID | AB_3669635 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P13726 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Normal blood coagulation is a complex process, involving a cascade of activation of different plasma proteins, ultimately resulting in the formation of a clot, called fibrin. Tissue Factor (TF), also named Coagulation factor III or F3, is the primary initiator of the blood coagulation cascade and plays an essential role in hemostasis (PMID: 26877187; PMID: 33796108).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Tissue Factor/CD142 antibody 28005-1-AP | Download protocol |
| WB protocol for Tissue Factor/CD142 antibody 28005-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Tissue Factor/CD142 Polyclonal antibody (Cat# 28005-1-AP) has 2 citations. They appear in Acta Pharmacol Sin and Genet Mol Biol. The cited studies cover research in pharmacology (pulmonary thromboembolism and extracellular vesicles) and molecular biology (tissue factor–mediated autophagy regulation in osteoclast differentiation). Both citations used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Acta Pharmacol Sin Lethal pulmonary thromboembolism in mice induced by intravenous human umbilical cord mesenchymal stem cell-derived large extracellular vesicles in a dose- and tissue factor-dependent manner | ||
Genet Mol Biol Tissue Factor-inhibited Beclin1-autophagy via BCL2 overexpression suppresses the differentiation of human monocytes into mature osteoclasts. |
