Tested Applications
| Positive IHC detected in | human lung tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse lung tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28118-1-AP targets MUC5B in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27886 Product name: Recombinant human MUC5B protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 5548-5716 aa of NM_002458 Sequence: MLSGDTQDPTVQCQEDACNNTTCPQGFEYKRVAGQCCGECVQTACLTPDGQPVQLNETWVNSHVDNCTVYLCEAEGGVHLLTPQPASCPDVSSCRGSLRKTGCCYSCEEDSCQVRINTTILWHQGCETEVNITFCEGSCPGASKYSAEAQAMQHQCTCCQERRVHEETVP Predict reactive species |
| Full Name | mucin 5B, oligomeric mucus/gel-forming |
| Calculated Molecular Weight | 596 kDa |
| GenBank Accession Number | NM_002458 |
| Gene Symbol | MUC5B |
| Gene ID (NCBI) | 727897 |
| RRID | AB_2881067 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HC84 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for MUC5B antibody 28118-1-AP | Download protocol |
| IHC protocol for MUC5B antibody 28118-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MUC5B polyclonal antibody (Cat# 28118-1-AP) has 10 citations across journals including Clinical and Translational Medicine, Frontiers in Immunology, Journal of Ethnopharmacology, Cells, Molecular Medicine Reports, Molecular Nutrition & Food Research, Journal of Pharmaceutical Sciences, PLoS ONE, and Veterinary Microbiology. Research fields span ovarian carcinoma, pulmonary fibrosis, innate immunity, chronic obstructive pulmonary disease, pyroptosis, mucus biology, mucin–drug interactions, and goblet cell differentiation.
| Species | Application | Title |
|---|---|---|
Clin Transl Med Single-cell transcriptomes reveal heterogeneity of high-grade serous ovarian carcinoma. | ||
Front Immunol Establishment of a porcine bronchial epithelial cell line and its application to study innate immunity in the respiratory epithelium | ||
J Ethnopharmacol Integrated pathological assessment and multi-omics analysis of the effects of Pinellia ternata aqueous extract on lung and spleen dysfunction in a rat model of phlegm-dampness obstruction. | ||
J Ethnopharmacol Synergistic effect of constituent drugs of Baibutang on improving Yin-deficiency pulmonary fibrosis in rats | ||
Cells Bleomycin-Induced Pulmonary Fibrosis in Transgenic Mice Carrying the Human MUC5B rs35705950 Variant | ||
Mol Med Rep Cigarette smoke combined with Klebsiella pneumoniae induce damage to the air‑blood barrier in chronic obstructive pulmonary disease rats via the MAPK/NF‑κB/IκBα pathway . |
