Tested Applications
| Positive WB detected in | mouse brain tissue, HeLa cells, rat brain tissue |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28284-1-AP targets DUSP3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28575 Product name: Recombinant human DUSP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-185 aa of BC002682 Sequence: MSGSFELSVQDLNDLLSDGSGCYSLPSQPCNEVTPRIYVGNASVAQDIPKLQKLGITHVLNAAEGRSFMHVNTNANFYKDSGITYLGIKANDTQEFNLSAYFERAADFIDQALAQKNGRVLVHCREGYSRSPTLVIAYLMMRQKMDVKSALSIVRQNREIGPNDGFLAQLCQLNDRLAKEGKLKP Predict reactive species |
| Full Name | dual specificity phosphatase 3 |
| Calculated Molecular Weight | 185 aa, 20 kDa |
| Observed Molecular Weight | 20 kDa |
| GenBank Accession Number | BC002682 |
| Gene Symbol | DUSP3 |
| Gene ID (NCBI) | 1845 |
| RRID | AB_2918154 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51452 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for DUSP3 antibody 28284-1-AP | Download protocol |
| WB protocol for DUSP3 antibody 28284-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DUSP3 antibody (Cat# 28284-1-AP) has 5 total citations. These appear in International Journal of Nanomedicine, Scientific Reports, Toxicology and Applied Pharmacology, Immunology and Inflammation Disease, and Tissue and Cell. Research fields span tissue engineering and wound repair, sepsis-induced myocardial dysfunction, oxidative stress and ferroptosis in cerebral ischemia, macrophage polarization in acute kidney injury, and hepatocellular carcinoma via cell senescence.
| Species | Application | Title |
|---|---|---|
Int J Nanomedicine USC-Derived Small Extracellular Vesicles-Functionalized Scaffolds Promote Scarless Vaginal Defect Repair via Delivery of Decorin and DUSP3 Proteins | ||
Sci Rep Piezo1 activation protects against sepsis-induced myocardial dysfunction in a pilot study | ||
Toxicol Appl Pharmacol Gastrodin inhibits the dephosphorylation of EGFR by DUSP3 to suppress oxidative stress-induced ferroptosis in astrocytes and alleviate cerebral ischemia-reperfusion injury. | ||
Immun Inflamm Dis Dehydroandrographolide facilitates M2 macrophage polarization by downregulating DUSP3 to inhibit sepsis-associated acute kidney injury | ||
Tissue Cell Targeting DUSP3 promotes cell senescence by activating the notch1 pathway to treat hepatocellular carcinoma
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