Tested Applications
| Positive WB detected in | Daudi cells, Ramos cells |
| Positive IHC detected in | human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28304-1-AP targets CD180 in WB, IHC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28476 Product name: Recombinant human CD180 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 164-389 aa of BC109069 Sequence: IKFPKDFPARNLKVLDFQNNAIHYISREDMRSLEQAINLSLNFNGNNVKGIELGAFDSTVFQSLNFGGTPNLSVIFNGLQNSTTQSLWLGTFEDIDDEDISSAMLKGLCEMSVESLNLQEHRFSDISSTTFQCFTQLQELDLTATHLKGLPSGMKGLNLLKKLVLSVNHFDQLCQISAANFPSLTHLYIRGNVKKLHLGVGCLEKLGNLQTLDLSHNDIEASDCCS Predict reactive species |
| Full Name | CD180 molecule |
| Calculated Molecular Weight | 661 aa, 74 kDa |
| Observed Molecular Weight | 95-105 kDa |
| GenBank Accession Number | BC109069 |
| Gene Symbol | CD180 |
| Gene ID (NCBI) | 4064 |
| RRID | AB_2881108 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99467 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for CD180 antibody 28304-1-AP | Download protocol |
| WB protocol for CD180 antibody 28304-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The CD180 Polyclonal antibody (Cat# 28304-1-AP) has a total of 1 citation. It appears in the journal Neural Regen Res (Impact Factor 8.5), published in 2026. The cited study investigates interferon regulatory factor 1's role in T cell differentiation in patients with myasthenia gravis, placing this citation within the fields of immunology and neurology.
| Species | Application | Title |
|---|---|---|
Neural Regen Res Interferon regulatory factor 1 enhances T cell differentiation in patients with myasthenia gravis. |
