RALGAPB Polyclonal antibody

RALGAPB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 28330-1-AP

Publications (4)

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Host / Isotype

Rabbit / IgG

Reactivity

Human, rat, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

KIAA1219, p170, RALGAPB

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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WB analysis using 28330-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28330-1-AP (RALGAPB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 28330-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 28330-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 28330-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 28330-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28330-1-AP (RALGAPB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (4)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, Jurkat cells, rat brain
Positive IHC detected in	human liver cancer tissue, mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28330-1-AP targets RALGAPB in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.

Tested Reactivity	Human, rat, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag28259 Product name: Recombinant human KIAA1219 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1222-1494 aa of BC047482 Sequence: NCCDDGEGSQQEVISSEDIGASIFNGQKKVLYYADALTEIAFVVPSPVESLTDSLESNISDQDSDSNMDLMPGILKQPSLTLELFPNHTDNLNSSQRLSPSSRMRKLPQGRPVPPLGPETRVSVVWVERYDDIENFPLSELMTEISTGVETTANSSTSLRSTTLEKEVPVIFIHPLNTGLFRIKIQGATGKFNMVIPLVDGMIVSRRALGFLVRQTVINICRRKRLESDSYSPPHVRRKQKITDIVNKYRNKQLEPEFYTSLFQEVGLKNCSS Predict reactive species
Full Name	KIAA1219
Observed Molecular Weight	160-170 kDa
GenBank Accession Number	BC047482
Gene Symbol	RALGAPB
Gene ID (NCBI)	57148
RRID	AB_2918155
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q86X10
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for RALGAPB antibody 28330-1-AP	Download protocol
WB protocol for RALGAPB antibody 28330-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The RALGAPB Polyclonal antibody (Cat# 28330-1-AP) has 4 total citations. These appear in Nature, Nature Communications, Life Science Alliance, and Biochemical and Biophysical Research Communications. The research fields span lipid metabolism and LDLR turnover, RalGAP tumor suppressor structure and mechanism, pancreatic disease involving secretion and primary cilia, and melanoma oncology related to circular RNA disruption of RalGAP complexes.

Species	Application	Title
		Nature Dietary cholesterol activates a Ral-dependent pathway driving LDLR turnover Authors - Xue Feng View Article
		Nat Commun Structure and mechanism of the RalGAP tumor suppressor complex Authors - René Rasche View Article
mouse	WB	Life Sci Alliance RalGAP complexes control secretion and primary cilia in pancreatic disease Authors - Lisa H Apken View Article
human	IHC	Biochem Biophys Res Commun Circ-ZBTB38 as an oncogenic circular RNA: Mechanisms in disrupting RalGAP complexes and its clinical value in melanoma. Authors - Wanqi Zhang View Article