Tested Applications
| Positive WB detected in | unboiled mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28423-1-AP targets PMCA2 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28994 Product name: Recombinant human PMCA2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 578-782 aa of NM_001001331 Sequence: LDLKQDYEPVRSQMPEEKLYKVYTFNSVRKSMSTVIKLPDESFRMYSKGASEIVLKKCCKILNGAGEPRVFRPRDRDEMVKKVIEPMACDGLRTICVAYRDFPSSPEPDWDNENDILNELTCICVVGIEDPVRPEVPEAIRKCQRAGITVRMVTGDNINTARAIAIKCGIIHPGEDFLCLEGKEFNRRIRNEKGEIEQERIDKIW Predict reactive species |
| Full Name | ATPase, Ca++ transporting, plasma membrane 2 |
| Calculated Molecular Weight | 137 kDa |
| Observed Molecular Weight | 137 kDa |
| GenBank Accession Number | NM_001001331 |
| Gene Symbol | PMCA2 |
| Gene ID (NCBI) | 491 |
| RRID | AB_3086051 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01814 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
ATP2B2, also named as PMCA2, belongs to the cation transport ATPase (P-type) family and type IIB subfamily. This magnesium-dependent enzyme catalyzes the hydrolysis of ATP coupled with the transport of calcium out of the cell.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for PMCA2 antibody 28423-1-AP | Download protocol |
| IHC protocol for PMCA2 antibody 28423-1-AP | Download protocol |
| WB protocol for PMCA2 antibody 28423-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The PMCA2 Polyclonal antibody (Cat# 28423-1-AP) has 3 citations published in Epigenomics, Tissue Cell, and J Proteomics. The associated research fields span DNA methylation in gallstone formation, immunolabel imaging of plasma membrane Ca²⁺ pumps in electrosensing fish, and hippocampal proteome analysis in Fmr1-deficient mice related to postsynaptic density changes. Cited applications include Western blot and immunofluorescence, with mouse as the cited reactive species.
| Species | Application | Title |
|---|---|---|
Tissue Cell Immunolabel imaging of plasma membrane Ca++ pumps (PMCA-pan or PMCA2) implicates the apical cilium on receptor cells as the transduction site for electrosense in two Acipenseriformes fishes | ||
J Proteomics Hippocampal proteome comparison of infant and adult Fmr1 deficiency mice reveals adult-related changes associated with postsynaptic density. |
Reviews
What customers say
The antibody received a 5-star rating. The reviewer reported it worked well for staining primary neuron cell cultures using immunofluorescence at a 1:500 dilution. Overall, the feedback was positive, indicating reliable performance in neural cell culture applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hanna (Verified Customer) (04-03-2024) | It works fine in staining of primary neuron cell culture.
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