DST Polyclonal antibody

DST Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 28438-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

DMH, D6S1101, CATX 15, BPAG1, BPA

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHCIF/ICC

WB analysis using 28438-1-AP
Mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 28438-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28438-1-AP (DST antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 28438-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 28438-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 28438-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver cancer using 28438-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 28438-1-AP (DST antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of SH-SY5Y using 28438-1-AP
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DST antibody (28438-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of SH-SY5Y using 28438-1-AP
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DST antibody (28438-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of SH-SY5Y using 28438-1-AP
Immunofluorescent analysis of (4% PFA) fixed SH-SY5Y cells using DST antibody (28438-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, rat brain tissue
Positive IHC detected in	mouse brain tissue, human liver cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	SH-SY5Y cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28438-1-AP targets DST in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag28833 Product name: Recombinant human DST protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 420-610 aa of NM_001144769 Sequence: SGVQFQNEAEIAGYILECENLLRQHVIDVQILIDGKYYQADQLVQRVAKLRDEIMALRNECSSVYSKGRILTTEQTKLMISGITQSLNSGFAQTLHPSLTSGLTQSLTPSLTSSSMTSGLSSGMTSRLTPSVTPAYTPGFPSGLVPNFSSGVEPNSLQTLKLMQIRKPLLKSSLLDQNLTEEEINMKFVQD Predict reactive species
Full Name	dystonin
Calculated Molecular Weight	861 kDa
GenBank Accession Number	NM_001144769
Gene Symbol	DST
Gene ID (NCBI)	667
RRID	AB_2881141
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q03001
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for DST antibody 28438-1-AP	Download protocol
IHC protocol for DST antibody 28438-1-AP	Download protocol
WB protocol for DST antibody 28438-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

DST Polyclonal antibody (catalog #28438-1-AP)

Total citation count: 1

The single citation appears in the Journal of Investigative Dermatology (PMID 39236903, 2024, Huang et al., titled "Paxbp1 is indispensable for the maintenance of epidermal homeostasis"). The research field associated with this citation is dermatology, specifically epidermal homeostasis. The study used the antibody for immunohistochemistry (IHC) in mouse samples.