Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, K-562 cells |
| Positive IP detected in | HEK-293T cells |
| Positive IF/ICC detected in | HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28502-1-AP targets DDX18 in WB, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27457 Product name: Recombinant human DDX18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 7-165 aa of BC001238 Sequence: KLLRKKIEKRNLKLRQRNLKFQGASNLTLSETQNGDVSEETMGSRKVKKSKQKPMNVGLSETQNGGMSQEAVGNIKVTKSPQKSTVLTNGEAAMQSSNSESKKKKKKKRKMVNDAEPDTKKAKTENKGKSEEESAETTKETENNVEKPDNDEDESEVPS Predict reactive species |
| Full Name | DEAD (Asp-Glu-Ala-Asp) box polypeptide 18 |
| Calculated Molecular Weight | 75 kDa |
| Observed Molecular Weight | 80-85 kDa |
| GenBank Accession Number | BC001238 |
| Gene Symbol | DDX18 |
| Gene ID (NCBI) | 8886 |
| RRID | AB_2881158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NVP1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for DDX18 antibody 28502-1-AP | Download protocol |
| IP protocol for DDX18 antibody 28502-1-AP | Download protocol |
| WB protocol for DDX18 antibody 28502-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DDX18 Polyclonal antibody (Cat# 28502-1-AP) has 4 citations. They appear in Journal of Clinical Investigation, Advanced Science, Journal of Radiation Research, and Nature Chemical Biology. The research fields span kidney fibrosis and lactylation, ovarian cancer platinum resistance, non-small cell lung cancer radiosensitivity and DNA damage repair, and nucleolar integrity/function.
| Species | Application | Title |
|---|---|---|
J Clin Invest Lactylation-dependent DDX18 nucleolar escape regulates CD44 mRNA stability to drive fibrotic progression in kidney injury. | ||
Adv Sci (Weinh) Ascites circRNA ASCOR Drives Platinum Resistance of High-Grade Serous Ovarian Cancer by Facilitating RPA1 Nuclear Translocation. | ||
J Radiat Res NOP58 modulates radiosensitivity in non-small cell lung cancer via DDX18-mediated DNA damage repair. | ||
Nat Chem Biol SRSF1 modulates the dark nucleolar cap pH to restore nucleolar integrity and function. |
Reviews
What customers say
The single review reports a 5-star rating for this antibody used in Western Blot at a 1:1000 dilution. The user fractionated Hek293 cells into nuclear and cytoplasmic fractions and observed DDX18 showing the expected nuclear localization, appearing just below the 100 kDa marker as anticipated. Overall, the reviewer found the antibody performed as expected for detecting DDX18 in their WB application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wael (Verified Customer) (12-08-2022) | Hek293 cells were fractionate into Nuclear and cytoplasmic fractions. DDX18 shows the expected Nuclear localization, just below the 100KD mark, as expected.
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