Tested Applications
| Positive WB detected in | HeLa cells, mouse brain tissue, mouse heart tissue |
| Positive IHC detected in | mouse brain tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28618-1-AP targets VPS13A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag29532 Product name: Recombinant human VPS13A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 2452-2595 aa of NM_033305 Sequence: PVGSRRLKWRCRKSHGEVTQKDDMMMPIDLGEKTIYLVSFFEGLQRIILFTEDPRVFKVTYESEKAELAEQEIAVALQDVGISLVNNYTKQEVAYIGITSSDVVWETKPKKKARWKPMSVKHTEKLEREFKEYTESSPSEDKVI Predict reactive species |
| Full Name | vacuolar protein sorting 13 homolog A (S. cerevisiae) |
| Calculated Molecular Weight | 360 kDa |
| Observed Molecular Weight | 360 kDa |
| GenBank Accession Number | NM_033305 |
| Gene Symbol | VPS13A |
| Gene ID (NCBI) | 23230 |
| RRID | AB_2918183 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96RL7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VPS13 (vacuolar protein sorting 13) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human: VPS13A, VPS13B, VPS13C, and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for VPS13A antibody 28618-1-AP | Download protocol |
| WB protocol for VPS13A antibody 28618-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
I'll search for citations for this antibody.VPS13A Polyclonal antibody (28618-1-AP) has a total of 6 citations. Journals include Cell Death Discovery, PNAS, PLoS Biology, International Journal of Molecular Sciences (2 papers), and bioRxiv. Research fields span reticulocyte maturation/lipidomics, plasma membrane repair, VPS13A/VPS13C lipid-transfer function in hematopoiesis and embryonic development, and the clinical characterization of Chorea-Acanthocytosis (VPS13A disease). Applications validated across these studies include Western blot and immunofluorescence, using human and mouse samples.
| Species | Application | Title |
|---|---|---|
Cell Death Discov Insights from lipidomics into the terminal maturation of circulating human reticulocytes | ||
Proc Natl Acad Sci U S A ATG9A-mediated plasma membrane repair is linked to Vps13A and regulated by glycosylation. | ||
PLoS Biol Impaired hematopoiesis and embryonic lethality at midgestation of mice lacking both lipid transfer proteins VPS13A and VPS13C.
| ||
Int J Mol Sci Analysis of Brain, Blood, and Testis Phenotypes Lacking the Vps13a Gene in C57BL/6N Mice | ||
Int J Mol Sci A Novel VPS13A Deletion in VPS13A Disease (Chorea-Acanthocytosis): A Case Report with Brief Literature Summary. | ||
bioRxiv Defect in hematopoiesis and embryonic lethality at midgestation of Vps13a/Vps13c double knockout mice
|
Reviews
What customers say
Able is busy answering your previous question. Please try again later.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Tom (Verified Customer) (09-27-2021) | Lysate from HEK293T - detects endogenous protein.
 |
