GREB1 Polyclonal antibody

GREB1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 28699-1-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

GREB1, GREB1 protein, KIAA0575, Protein GREB1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

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Filter: AllWBIHCIF/ICC

WB analysis using 28699-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 28699-1-AP
MCF-7 cells were subjected to SDS PAGE followed by western blot with 28699-1-AP (GREB1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 28699-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 28699-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 28699-1-AP (GREB1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 28699-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HepG2 using 28699-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using GREB1 antibody (28699-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (3)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, MCF-7 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28699-1-AP targets GREB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity	Human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag29908 Product name: Recombinant human GREB1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1075-1212 aa of NM_014668 Sequence: EVPLEKGARNEALESDAEKLSSTDNEDEELGTEGSTSEKRSPMKRERSRSHDSASSSLSSKASGSALGGESSAQPTALPQGEHARSPQPRGPAEEGRAPGEKQRPRASQGPPSAISRHSPGPTPQPDCSLRTGQRSVQ Predict reactive species
Full Name	GREB1 protein
Calculated Molecular Weight	216 kDa
Observed Molecular Weight	216-240 kDa
GenBank Accession Number	NM_014668
Gene Symbol	GREB1
Gene ID (NCBI)	9687
RRID	AB_2881196
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q4ZG55
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GREB1 (Gene regulated by estrogen in breast cancer protein1) encodes a 1949-amino acid protein that contains an N-terminal Zn-binding domain followed by a circularly permuted catalytically inactive superfamily II helicase domain and a C-terminal Fringe-like GT domain. GREB1 is an early estrogen-responsive gene, and its expression is correlated with estrogen levels in breast cancer patients. GREB1 is implicated in hormone-dependent cancers, such as ovarian, prostate cancer and as a risk factor in endometriosis and variations in bone mineral density.

Protocols

Product Specific Protocols
IF protocol for GREB1 antibody 28699-1-AP	Download protocol
IHC protocol for GREB1 antibody 28699-1-AP	Download protocol
WB protocol for GREB1 antibody 28699-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
		Cancer Gene Ther Tafazzin mediates tamoxifen resistance by regulating cellular phospholipid composition in ER-positive breast cancer Authors - Xuan Li View Article
human	WB	Cancers (Basel) Pharmacological Inhibition of CBP/p300 Blocks Estrogen Receptor Alpha (ERα) Function through Suppressing Enhancer H3K27 Acetylation in Luminal Breast Cancer. Authors - Aaron Waddell View Article
human	WB	Exp Cell Res EFHD1 promotes breast cancer progression through estrogen signaling Authors - Xiaojun Sun View Article