Tested Applications
| Positive WB detected in | mouse heart tissue, rat heart tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29023-1-AP targets DKK2 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30596 Product name: Recombinant human DKK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 34-183 aa of BC075078 Sequence: KLNSIKSSLGGETPGQAANRSAGMYQGLAFGGSKKGKNLGQAYPCSSDKECEVGRYCHSPHQGSSACMVCRRKKKRCHRDGMCCPSTRCNNGICIPVTESILTPHIPALDGTRHRDRNHGHYSNHDLGWQNLGRPHTKMSHIKGHEGDPC Predict reactive species |
| Full Name | dickkopf homolog 2 (Xenopus laevis) |
| Calculated Molecular Weight | 259 aa, 28 kDa |
| Observed Molecular Weight | 28 kDa |
| GenBank Accession Number | BC075078 |
| Gene Symbol | DKK2 |
| Gene ID (NCBI) | 27123 |
| RRID | AB_3086095 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UBU2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for DKK2 antibody 29023-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The DKK2 Polyclonal antibody (Cat# 29023-1-AP) has 3 citations published in Life Sciences, Scientific Reports, and Journal of Molecular Histology. The research fields span Wnt/β-catenin signaling, high-fat diet-induced myocardial injury, osteogenic differentiation, and exosomal miRNA regulation in bone mesenchymal stem cells. Applications cited include WB and IHC across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Life Sci Downregulated DKK2 may serve as a molecular mechanism of high-fat diet-induced myocardial injury via Wnt/β-catenin pathway | ||
Sci Rep Bone mesenchymal stem cells-derived exosomal miR-509-5p promotes osteogenic differentiation by targeting SFRP1 and activating the Wnt/β-catenin pathway. | ||
J Mol Histol QiangGuYin-containing serum improves osteogenic differentiation via regulating osteoclast-derived exosomal miR-27b-3p. |
