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GANAB Polyclonal antibody

GANAB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 29183-1-AP

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

glucosidase, alpha; neutral AB, Glucosidase II subunit alpha, G2AN, EC:3.2.1.207, Alpha-glucosidase 2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIHC

WB analysis using 29183-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 29183-1-AP (GANAB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HeLa using 29183-1-AP
WB result of GANAB antibody (29183-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-GANAB transfected HeLa cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon using 29183-1-AP
Immunohistochemical analysis of paraffin-embedded human normal colon slide using 29183-1-AP (GANAB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 29183-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29183-1-AP (GANAB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse liver using 29183-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 29183-1-AP (GANAB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse kidney tissue, HeLa cells, mouse liver, rat liver
Positive IHC detected in	mouse liver tissue, human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29183-1-AP targets GANAB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag30417 Product name: Recombinant human GANAB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 350-488 aa of BC017435 Sequence: GGTIVPRWMRVRRSSECMKDDPITLFVALSPQGTAQGELFLDDGHTFNYQTRQEFLLRRFSFSGNTLVSSSADPEGHFETPIWIERVVIIGAGKPAAVVLQTKGSPESRLSFQHDPETSVLVLRKPGINVASDWSIHLR Predict reactive species
Full Name	glucosidase, alpha; neutral AB
Calculated Molecular Weight	944aa,107 kDa; 488aa,55 kDa
Observed Molecular Weight	110-120 kDa
GenBank Accession Number	BC017435
Gene Symbol	GANAB
Gene ID (NCBI)	23193
RRID	AB_2918244
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q14697
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for GANAB antibody 29183-1-AP	Download protocol
WB protocol for GANAB antibody 29183-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The GANAB Polyclonal antibody (Cat# 29183-1-AP) has 1 citation. It appears in Glycobiology (2017), where it was used in Western blot to study MeCP2-regulated glycogenes contributing to proliferation and apoptosis of gastric cancer cells. The research field is gastric cancer biology.

Species	Application	Title
human	WB	Glycobiology MeCP2 regulated glycogenes contribute to proliferation and apoptosis of gastric cancer cells. Authors - Yannan Qin KD Validated View Article