Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, mouse lung tissue |
| Positive IHC detected in | mouse heart tissue, mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29392-1-AP targets LMO7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30016 Product name: Recombinant human LMO7 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 695-838 aa of NM_005358 Sequence: KIYGENGSKSMSDVSAEDVQNLRQLRYEEMQKIKSQLKEQDQKWQDDLAKWKDRRKSYTSDLQKKKEEREEIEKQALEKSKRSSKTFKEMLQDRESQNQKSTVPSRRRMYSFDDVLEEGKRPPTMTVSEASYQSERVEEKGATY Predict reactive species |
| Full Name | LIM domain 7 |
| Calculated Molecular Weight | 193 kDa |
| Observed Molecular Weight | 140-160 kDa |
| GenBank Accession Number | NM_005358 |
| Gene Symbol | LMO7 |
| Gene ID (NCBI) | 4008 |
| RRID | AB_3086128 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8WWI1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LMO7, also known as LOMP, FBX20, belongs to the single Calponin-homology (CH) domain-containing protein family, with a single actin-binding CH domain at the amino terminal end(PMID: 19459066). LMO7 also contains a PDZ domain and a carboxyl-terminal LIM domain, both of which have been described as protein-protein interacting domains(PMID: 15140894). LMO7 encodes three splice variants of calculated molecular weights of 196 kDa (P200), 157 kDa (P150), and 100 kDa (P100)(PMID: 26157009).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LMO7 antibody 29392-1-AP | Download protocol |
| IHC protocol for LMO7 antibody 29392-1-AP | Download protocol |
| WB protocol for LMO7 antibody 29392-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LMO7 Polyclonal antibody (Cat# 29392-1-AP) has 1 total citation. It was cited in BMC Cancer in a 2025 study on SUN modification-related anti-PD-1 immunotherapy-resistance signatures for predicting prognosis and immune microenvironment status in glioblastoma. The research field encompasses glioblastoma oncology, immunotherapy resistance mechanisms, and tumor immune microenvironment analysis. The antibody was applied in Western Blot (WB) using human samples.
| Species | Application | Title |
|---|---|---|
BMC Cancer Identification and validation of SUN modification-related anti-PD-1 immunotherapy-resistance signatures to predict prognosis and immune microenvironment status in glioblastoma. |
