Tested Applications
| Positive WB detected in | HEK-293T cells, MCF-7 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse eye tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29394-1-AP targets TPBG in WB, IHC, ELISA applications and shows reactivity with Human, rat, mouse samples.
| Tested Reactivity | Human, rat, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30019 Product name: Recombinant human TPBG protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 153-355 aa of NM_006670 Sequence: PLADLSPFAFSGSNASVSAPSPLVELILNHIVPPEDERQNRSFEGMVVAALLAGRALQGLRRLELASNHFLYLPRDVLAQLPSLRHLDLSNNSLVSLTYVSFRNLTHLESLHLEDNALKVLHNGTLAELQGLPHIRVFLDNNPWVCDCHMADMVTWLKETEVVQGKDRLTCAYPEKMRNRVLLELNSADLDCDPILPPSLQTS Predict reactive species |
| Full Name | trophoblast glycoprotein |
| Calculated Molecular Weight | 46 kDa |
| Observed Molecular Weight | 72-80 kDa |
| GenBank Accession Number | NM_006670 |
| Gene Symbol | TPBG |
| Gene ID (NCBI) | 7162 |
| RRID | AB_2918290 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13641 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Trophoblast glycoprotein (TPBG), also termed 5T4, is a 72 kDa transmembrane glycoprotein and an extensively investigated oncofetal antigen, which is limited in normal adult tissues, but highly expressed in various types of cancer in human. TPBG is a novel PKCa-dependent phosphoprotein in retinal rod bipolar cells (RBCs). (PMID: 18097562, PMID: 31255707)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TPBG antibody 29394-1-AP | Download protocol |
| WB protocol for TPBG antibody 29394-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TPBG polyclonal antibody (Cat# 29394-1-AP) has 2 citations. These appear in Journal of Cellular and Molecular Medicine (2025), examining TPBG as a predictive indicator for gastric cancer using WB, and Cancer Management and Research (2022), investigating MIR31HG in colorectal cancer progression using IHC. Both studies focus on human samples and relate to oncology research, specifically gastric and colorectal cancer biomarker discovery and prognostic evaluation.
| Species | Application | Title |
|---|---|---|
J Cell Mol Med In-Depth Examination of TPBG as a New Predictive Indicator for Gastric Cancer | ||
Cancer Manag Res MIR31HG Expression Predicts Poor Prognosis and Promotes Colorectal Cancer Progression. |
