Tested Applications
| Positive WB detected in | A549 cells, Hela cells, Jurkat cells, K-562 cells, MCF-7 cells |
| Positive IHC detected in | human stomach cancer tissue, mouse stomach tissue, rat stomach tissue, mouse testis tissue, human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29603-1-AP targets BICD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31279 Product name: Recombinant human BICD2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 361-523 aa of BC073970 Sequence: GLLATLQDTQKQLEHTRGSLSEQQEKVTRLTENLSALRRLQASKERQTALDNEKDRDSHEDGDYYEVDINGPEILACKYHVAVAEAGELREQLKALRSTHEAREAQHAEEKGRYEAEGQALTEKVSLLEKASRQDRELLARLEKELKKVSDVAGETQGSLSVA Predict reactive species |
| Full Name | bicaudal D homolog 2 (Drosophila) |
| Calculated Molecular Weight | 855 aa, 97 kDa |
| Observed Molecular Weight | 93-96 kDa |
| GenBank Accession Number | BC073970 |
| Gene Symbol | BICD2 |
| Gene ID (NCBI) | 23299 |
| RRID | AB_2935474 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8TD16 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Protein bicaudal D homolog 2 (BICD2) acts as an adapter protein linking the dynein motor complex to various cargos and converts dynein from a non-processive to a highly processive motor in the presence of dynactin. Facilitates and stabilizes the interaction between dynein and dynactin and activates dynein processivity (the ability to move along a microtubule for a long distance without falling off the track). Facilitates the binding of RAB6A to the Golgi by stabilizing its GTP-bound form. Regulates coat complex coatomer protein I (COPI)-independent Golgi-endoplasmic reticulum transport via its interaction with RAB6A and recruitment of the dynein-dynactin motor complex (PubMed:25962623).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for BICD2 antibody 29603-1-AP | Download protocol |
| WB protocol for BICD2 antibody 29603-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BICD2 Polyclonal antibody (Cat# 29603-1-AP) has 2 total citations. These appear in Advanced Science (IF 14.1) and Cell Reports (IF 7.7). The research fields span RNA modification (programmable m6,2⁸O-dimethyladenosine editing) and intracellular transport (COP9 signalosome/PRMT5 methylosome regulation of Lis1-dynein-based transport). Both studies used the antibody for Western blot in human samples.
| Species | Application | Title |
|---|---|---|
Cell Rep COP9 signalosome and PRMT5 methylosome complexes are essential regulators of Lis1-dynein-based transport. |
