Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29654-1-AP targets FRAS1 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31229 Product name: Recombinant human FRAS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 242-388 aa of NM_025074 Sequence: CICDRGEVRCHKQACLPLRCGKGQSRARRHGQCCEECVSPAGSCSYDGVVRYQDEMWKGSACEFCMCDHGQVTCQTGECAKVECARDEELIHLDGKCCPECISRNGYCVYEETGEFMSSNASEVKRIPEGEKWEDGPCKVCECRGAQ Predict reactive species |
| Full Name | Fraser syndrome 1 |
| Calculated Molecular Weight | 443 kDa |
| Observed Molecular Weight | 450-500 kDa |
| GenBank Accession Number | NM_025074 |
| Gene Symbol | FRAS1 |
| Gene ID (NCBI) | 80144 |
| RRID | AB_3086149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86XX4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FRAS1 (extracellular matrix organizing protein FRAS1) is required for the regulation of epidermal-basement membrane adhesion responsible for proper organogenesis during embryonic development, and it is also involved in brain organization and function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FRAS1 antibody 29654-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review rates this antibody 5 stars for Western Blot. The user successfully detected the large FRAS1 protein in prostate cancer cell lysates (DU145 and 22RV1) at a 1:5000 dilution. Specificity was confirmed by comparing parental cells against FRAS1 knockout and knockdown lines. The reviewer described the antibody as excellent and highly recommended it for FRAS1 detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Evangelos (Verified Customer) (06-17-2024) | Excellent antibody for detecting the very large FRAS1 protein in western blot! We used it at a dilution of 1:5000, to detect FRAS1 in prostate cancer cell lysates and woked out great. We confirmed specificity by comparing parental vs. FRAS1 knockout and knock down cells. Highly recomend it
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