Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human ovary cancer tissue, human intrahepatic cholangiocarcinoma tissue, mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29775-1-AP targets Integrin beta-8 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31137 Product name: Recombinant human Integrin beta-8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 460-620 aa of NM_002214 Sequence: KIHIHRNCSCQCEDNRGPKGKCVDETFLDSKCFQCDENKCHFDEDQFSSESCKSHKDQPVCSGRGVCVCGKCSCHKIKLGKVYGKYCEKDDFSCPYHHGNLCAGHGECEAGRCQCFSGWEGDRCQCPSAAAQHCVNSKGQVCSGRGTCVCGRCECTDPRSI Predict reactive species |
| Full Name | integrin, beta 8 |
| Calculated Molecular Weight | 86 kDa |
| Observed Molecular Weight | 95 kDa |
| GenBank Accession Number | NM_002214 |
| Gene Symbol | Integrin beta 8 |
| Gene ID (NCBI) | 3696 |
| RRID | AB_2935479 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P26012 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Integrins are cell adhesion receptors that are heterodimers composed of non-covalently associated alpha and beta subunits. Integrin beta-8 (ITGB8), belongs to the integrin beta chain family, contains an N-terminal signal peptide, a large extracellular domain that includes four cysteine-rich repeats, a transmembrane domain, and a C-terminal cytoplasmic domain (PMID: 1918072). Integrin beta-8 associates with integrin alpha-V (ITGAV) to form an αvβ8 heterodimer which has been shown to bind vitronectin, fibronectin, and a latency-associated peptide of TGF-β1/3 (PMID: 27033701). Integrin beta-8 contains multiple potential N-linked glycosylation sites. The observed molecular mass of approximately 95 kDa for integrin beta-8 is larger than the predicted 86 kDa, consistent with substantial glycosylation (PMID: 1918072).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for Integrin beta-8 antibody 29775-1-AP | Download protocol |
| WB protocol for Integrin beta-8 antibody 29775-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Integrin beta-8 Polyclonal antibody (Cat# 29775-1-AP) has 4 total citations. These appear in Nature Communications, Experimental & Molecular Medicine, Science Advances, and Medicine (Baltimore). The cited research spans immunology (oral tolerance via innate sensing pathways), bone biology (osteoclastogenesis and mitochondrial respiration), and oncology (colorectal cancer metastasis and hepatocellular carcinoma).
| Species | Application | Title |
|---|---|---|
Nat Commun Dietary nucleic acids promote oral tolerance through innate sensing pathways in mice | ||
Exp Mol Med Osteoblast-derived osteomodulin restrains osteoclastogenesis via ITGB8/RRM2-mediated reduction of mitochondrial respiration and mitochondrial ATP production. | ||
Sci Adv AKR1B10 dictates c-Myc stability to suppress colorectal cancer metastasis via PP2A nitration | ||
Medicine (Baltimore) Prognostic value and potential molecular mechanism of ITGB superfamily members in hepatocellular carcinoma |
