Tested Applications
| Positive WB detected in | HeLa cells, mouse testis tissue, HEK-293 cells, Jurkat cells, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29778-1-AP targets RPGRIP1L in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse, zebrafish |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31168 Product name: Recombinant human RPGRIP1L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-140 aa of NM_015272 Sequence: MSGPTDETAGDLPVKDTGLNLFGMGGLQETSTTRTMKSRQAVSRVSREELEDRFLRLHDENILLKQHARKQEDKIKRMATKLIRLVNDKKRYERVGGGPKRLGRDVEMEEMIEQLQEKVHELEKQNETLKNRLISAKQQL Predict reactive species |
| Full Name | RPGRIP1-like |
| Calculated Molecular Weight | 151 kDa |
| Observed Molecular Weight | 151 kDa |
| GenBank Accession Number | NM_015272 |
| Gene Symbol | RPGRIP1L |
| Gene ID (NCBI) | 23322 |
| RRID | AB_2923607 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q68CZ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RPGRIP1L, also named as FTM, KIAA1005, belongs to the RPGRIP1 family. It negatively regulates signaling through the G-protein coupled thromboxane A2 receptor. RPGRIP1L may be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis.(PMID:17558409) Defects in RPGRIP1L are the cause of Joubert syndrome type 7 (JBTS7). Defects in RPGRIP1L are the cause of Meckel syndrome type 5 (MKS5).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for RPGRIP1L antibody 29778-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPGRIP1L Polyclonal antibody (Cat# 29778-1-AP) has 5 total citations published in Cell Mol Neurobiol, Dis Model Mech, bioRxiv, eLife, and Nat Commun. The associated research fields span neurobiology (behavioral phenotypes in SUMO1 knockout mice), ciliogenesis and ciliary transition zone biology, Meckel-Gruber syndrome/Wnt signaling, centriolar satellite function, and phase-separated condensate assembly in cilia.
| Species | Application | Title |
|---|---|---|
Cell Mol Neurobiol Downregulation of Hmox1 and Rpgrip1l Expression Linked to Risk-Taking Behavior, Reduced Depressive Symptoms, and Diminished Novelty Socialization in SUMO1 Knockout Mice | ||
Dis Model Mech Variable phenotypes and penetrance between and within different zebrafish ciliary transition zone mutants | ||
bioRxiv Two functional forms of the Meckel-Gruber syndrome protein TMEM67 generated by proteolytic cleavage by ADAMTS9 mediate Wnt signaling and ciliogenesis | ||
Nat Commun CFAP100 forms phase-separated condensates required for ciliary transition zone assembly. |
