Tested Applications
| Positive WB detected in | A431 cells |
| Positive IHC detected in | mouse embryo tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29781-1-AP targets SCPEP1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31396 Product name: Recombinant human SCPEP1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 221-452 aa of BC072405 Sequence: LLEDKGLAEVSKVAEQVLNAVNKGLYREATELWGKAEMIIEQNTDGVNFYNILTKSTPTSTMESSLEFTQSHLVCLCQRHVRHLQRDALSQLMNGPIRKKLKIIPEDQSWGGQATNVFVNMEEDFMKPVISIVDELLEAGINVTVYNGQLDLIVDTMGQEAWVRKLKWPELPKFSQLKWKALYSDPKSLETSAFVKSYKNLAFYWILKAGHMVPSDQGDMALKMMRLVTQQE Predict reactive species |
| Full Name | serine carboxypeptidase 1 |
| Calculated Molecular Weight | 452 aa, 51 kDa |
| Observed Molecular Weight | 30 kDa, 51 kDa |
| GenBank Accession Number | BC072405 |
| Gene Symbol | SCPEP1 |
| Gene ID (NCBI) | 59342 |
| RRID | AB_2923608 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HB40 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SCPEP1 is synthesized as a ~51-55 kDa precursor (zymogen) containing a signal peptide and five N-glycosylation sites. During lysosomal maturation, it undergoes proteolytic cleavage and N-glycan trimming, producing multiple forms that can appear at ~30 kDa on WB. (PMID: 19187242, 16461364)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SCPEP1 antibody 29781-1-AP | Download protocol |
| WB protocol for SCPEP1 antibody 29781-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SCPEP1 Polyclonal antibody (Cat# 29781-1-AP) has 1 total citation. It was cited in Frontiers in Immunology (2025), in a study examining SCPEP1-positive basal cells and their association with remodeling of oxidative stress signaling networks in idiopathic pulmonary fibrosis. The research field encompasses pulmonary fibrosis, oxidative stress biology, and immunology. The antibody was applied via Western blot in mouse samples.
| Species | Application | Title |
|---|---|---|
Front Immunol SCPEP1(+) basal cells are associated with the remodeling of oxidative stress signaling networks in idiopathic pulmonary fibrosis. |
