Tested Applications
| Positive WB detected in | A549 cells, HepG2 cells, U-251 cells, U-87 MG cells, mouse testis tissue, rat testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29820-1-AP targets BAG2 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30873 Product name: Recombinant human BAG2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-134 aa of NM_004282 Sequence: MAQAKINAKANEGRFCRSSSMADRSSRLLESLDQLELRVEALREAATAVEQEKEILLEMIHSIQNSQDMRQISDGEREELNLTANRLMGRTLTVEVSVETIRNPQQQESLKHATRIIDEVVNKFLDDLGNAKSH Predict reactive species |
| Full Name | BCL2-associated athanogene 2 |
| Calculated Molecular Weight | 24 kDa |
| Observed Molecular Weight | 25-27 kDa |
| GenBank Accession Number | NM_004282 |
| Gene Symbol | BAG2 |
| Gene ID (NCBI) | 9532 |
| RRID | AB_2918348 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95816 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
BAG2 (BAG family molecular chaperone regulator 2) is one of six proteins in mammals that contain the BAG domain, which belongs to the BAG (Bcl-2-associated athanogene) family. BAG2 has been described as a negative regulator of the chaperone-associated ubiquitin ligase C terminus of Hsc70-interacting protein (CHIP) that participates in the ubiquitin-mediated proteasomal degradation of misfolded substrate proteins (PMID: 16207813). BAG2 is widely expressed in human tissues, including brown adipose, heart and lung tissue, as well as in various types of tumor cells, including renal cell carcinoma, glioblastoma and thyroid carcinoma cells (PMID: 28536620). BAG2 overexpression is associated with poor prognosis in patients and mutp53 accumulation in tumors (PMID: 26271008).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for BAG2 antibody 29820-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The BAG2 Polyclonal antibody (Cat# 29820-1-AP) has 2 total citations. These appear in PLoS Pathogens and iScience. The cited research spans virology and infectious disease (host-specific mutations in hepatitis A virus 3Dpol polymerase) and cell biology/proteomics (proximity labeling of inter-organelle membrane contacts). Both citations used the antibody for Western blot applications in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
PLoS Pathog Host species-specific mutations in the thumb domain of the 3Dpol polymerase are required for efficient replication of human hepatitis A virus in mice.
| ||
iScience A proximity labeling strategy enables proteomic analysis of inter-organelle membrane contacts |
