Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29844-1-AP targets VPS13C in WB, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31069 Product name: Recombinant human VPS13C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 95-253 aa of NM_017684 Sequence: SIKYDAVKEEKSLQDVKQKELSRIEEALQKAAEKGTHSGEFIYGLENFVYKDIKPGRKRKKHKKHFKKPFKGLDRSKDKPKEAKKDTFVEKLATQVIKNVQVKITDIHIKYEDDVTDPKRPLSFGVTLGELSLLTANEHWTPCILNEADKIIYKLIRLD Predict reactive species |
| Full Name | vacuolar protein sorting 13 homolog C (S. cerevisiae) |
| Calculated Molecular Weight | 422 kDa |
| Observed Molecular Weight | 422 kDa |
| GenBank Accession Number | NM_017684 |
| Gene Symbol | VPS13C |
| Gene ID (NCBI) | 54832 |
| RRID | AB_3086177 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q709C8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
VPS13C (vacuolar protein sorting 13 homolog C) acts at membrane contact sites between intracellular organelles to transport lipids. There are four VPS13 family members in human - VPS13A, VPS13B, VPS13C, and VPS13D. Mutations in human VPS13 genes are linked to rare neurodegenerative disorders: chorea- acanthocytosis (VPS13A), Cohen syndrome (VPS13B), predispose to early onset into Parkinson's disease (VPS13C) and lead to ataxia/spastic paraplegia (VPS13D) (PMID: 30656912).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VPS13C antibody 29844-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The VPS13C Polyclonal antibody (Cat# 29844-1-AP) has 4 total citations published in Nature Cell Biology, Molecular Cell, NPJ Parkinson's Disease, and bioRxiv. The research spans lipid transport at ER-lysosome membrane contacts, cryo-EM structural biology of VPS13C, and Parkinson's disease biomarker evaluation in large animal models. Applications cited include Western blot and immunofluorescence in human and pig samples.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol The bridge-like lipid transport protein VPS13C/PARK23 mediates ER-lysosome contacts following lysosome damage | ||
Mol Cell Cryo-EM structure of soluble VPS13C suggests its regulation by a conformational switch and by calmodulin. | ||
NPJ Parkinsons Dis Evaluating Parkinson's disease biomarkers in substantia nigra following sublethal γ-radiation exposure in a large animal model. | ||
bioRxiv Lysosome damage triggers acute formation of ER to lysosomes membrane tethers mediated by the bridge-like lipid transport protein VPS13C |
