Tested Applications
| Positive WB detected in | mouse colon tissue, A549 cells |
| Positive IHC detected in | human cervical cancer tissue, human ovary tumor tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29921-1-AP targets OTUD1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31996 Product name: Recombinant human OTUD1 protein Source: e coli.-derived, PGEX-4T Tag: GST Sequence: SSAAEPVIVSRSDPRDEKLALYLAEVEKQDKYLRQRNKYRFHIIPDGNCLYRAVSKTVYGDQSLHRELREQTVHYIADHLDHFSPLIEGDVGEFIIAAAQDGAWAGYPELLAMGQMLNVNIHLTTGGRLESPTVSTMIHYLGPEDSLRPSIWLSWLSNGH Predict reactive species |
| Full Name | OTU domain containing 1 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 50-55 kDa |
| Gene Symbol | OTUD1 |
| Gene ID (NCBI) | 220213 |
| RRID | AB_3086190 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VV17 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
OTUD1, OTU domain-containing protein 1, is a deubiquitinating enzyme that specifically hydrolyzes 'Lys-63'-linked polyubiquitin to monoubiquitin (PMID: 23827681). OTUD1-mediated deubiquitination prevents activation of the ribosome quality control and subsequent dissociation of ribosomes and stimulates formation of polysomes and translation (PMID: 36445135). OTUD1 is a colon-specific deubiquitinase that expressed in normal colonic epithelium goblet cells (PMID: 36910614).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for OTUD1 antibody 29921-1-AP | Download protocol |
| WB protocol for OTUD1 antibody 29921-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-OTUD1 antibody (Cat# 29921-1-AP) has 8 total citations published in journals including Nature Communications, Cell Death & Disease, Cell Molecular Biology Letters, Molecular Medicine Reports, Molecular Human Reproduction, Frontiers in Bioscience, International Journal of Cardiology, and Molecular Biomedicine. Research fields span ovarian cancer, esophageal squamous cell carcinoma, neonatal lung injury, COPD, endometriosis fibrosis, heart failure, myocardial infarction/reperfusion injury, and steatotic liver disease.
| Species | Application | Title |
|---|---|---|
Nat Commun Aggresome formation promotes ASK1/JNK signaling activation and stemness maintenance in ovarian cancer | ||
Cell Mol Biol Lett OTULIN protects hyperoxia-induced neonatal lung injury and modulates mitochondrial protein OPA1 in association with the E3 ubiquitin ligase RNF31. | ||
Cell Death Dis HOXC9 accelerates esophageal squamous cell carcinoma progression via OTUD1-FABP5-mediated lipid metabolic reprogramming. | ||
Mol Med Rep METTL3‑mediated m6A methylation and its impact on OTUD1 expression in chronic obstructive pulmonary disease | ||
Front Biosci (Landmark Ed) VPO1 Promotes Programmed Necrosis of Cardiomyocytes in Rats with Chronic Heart Failure by Upregulating CYLD |
