Tested Applications
| Positive WB detected in | A549 cells, HEK-293T cells, HT-29 cells, HeLa cells, Jurkat cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29935-1-AP targets EIF2B5 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Reactivity | Human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag32175 Product name: Recombinant human EIF2B5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 455-711 aa of NM_003907.2 Sequence: DAEEDEDDGEFSDDSGADQEKDKVKMKGYNPAEVGAAGKGYLWKAAGMNMEEEEELQQNLWGLKINMEEESESESEQSMDSEEPDSRGGSPQMDDIKVFQNEVLGTLQRGKEENISCDNLVLEINSLKYAYNISLKEVMQVLSHVVLEFPLQQMDSPLDSSRYCALLLPLLKAWSPVFRNYIKRAADHLEALAAIEDFFLEHEALGISMAKVLMAFYQLEILAEETILSWFSQRDTTDKGQQLRKNQQLQRFIQWLK Predict reactive species |
| Full Name | eukaryotic translation initiation factor 2B, subunit 5 epsilon, 82kDa |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 65-80 kDa |
| GenBank Accession Number | NM_003907.2 |
| Gene Symbol | EIF2B5 |
| Gene ID (NCBI) | 8893 |
| RRID | AB_2923623 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13144 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
EIF2B5 encodes translation initiation factor eIF-2B subunit epsilon protein (PMID 8688466). EIF2B5 mutations compromise the induction of GFAP-expressing astrocytes in VWM (vanishing white matter) leukodystrophy (PMID:15723074, PMID:15776425). The upregulation of EIF2B5 is associated with cancer such as human pulmonary adenocarcinoma cell and breast cancer (PMID:24366584, PMID:24979338). EIF2B5 also has 65kDa truncated protein isoform (PMID:28961236).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EIF2B5 antibody 29935-1-AP | Download protocol |
| IHC protocol for EIF2B5 antibody 29935-1-AP | Download protocol |
| WB protocol for EIF2B5 antibody 29935-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The EIF2B5 Polyclonal antibody (Cat# 29935-1-AP) has 1 citation. It appears in Cell Signal (2025), in a study demonstrating that EIF2B5 promotes malignant progression of hepatocellular carcinoma by activating the PI3K/AKT signaling pathway through targeting RPL6. The associated research field is liver cancer (hepatocellular carcinoma) and oncogenic signaling pathway mechanisms.
| Species | Application | Title |
|---|---|---|
Cell Signal EIF2B5 promotes malignant progression of hepatocellular carcinoma by activating the PI3K/AKT signaling pathway through targeting RPL6 |
